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  Support and challenges to the melanosomal casing model based on nanoscale distribution of metals within iris melanosomes detected by X-ray fluorescence analysis

Gorniak, T., Haraszti, T., Suhonen, H., Yang, Y., Hedberg-Buenz, A., Koehn, D., et al. (2014). Support and challenges to the melanosomal casing model based on nanoscale distribution of metals within iris melanosomes detected by X-ray fluorescence analysis. Pigment Cell & Melanoma Research, 27(5), 831-834. doi:10.1111/pcmr.12278.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-5F72-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-7EA6-8
Genre: Journal Article

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 Creators:
Gorniak, Thomas, Author
Haraszti, Tamas1, 2, Author              
Suhonen, Heikki, Author
Yang, Yang, Author
Hedberg-Buenz, Adam, Author
Koehn, Demelza, Author
Heine, Ruth, Author
Grunze, Michael1, Author              
Rosenhahn, Axel, Author
Anderson, Michael G., Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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Free keywords: X-ray fluorescence analysis; eumelanin; melanosome; metal; pheomelanin; synchrotron-based imaging
 Abstract: Melanin within melanosomes exists as eumelanin or pheomelanin. Distributions of these melanins have been studied extensively within tissues, but less often within individual melanosomes. Here, we apply X-ray fluorescence analysis with synchrotron radiation to survey the nanoscale distribution of metals within purified melanosomes of mice. The study allows a discovery-based characterization of melanosomal metals, and, because Cu is specifically associated with eumelanin, a hypothesis-based test of the 'casing model' predicting that melanosomes contain a pheomelanin core surrounded by a eumelanin shell. Analysis of Cu, Ca, and Zn shows variable concentrations and distributions, with Ca/Zn highly correlated, and at least three discrete patterns for the distribution of Cu vs. Ca/Zn in different melanosomes - including one with a Cu-rich shell surrounding a Ca/Zn-rich core. Thus, the results support predictions of the casing model, but also suggest that in at least some tissues and genetic contexts, other arrangements of melanin may co-exist.

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Language(s): eng - English
 Dates: 2014-05-032014-04-042014-05-032014-06-052014-09-01
 Publication Status: Published in print
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Pigment Cell & Melanoma Research
  Other : (Formerly: Pigment Cell Research)
  Abbreviation : Pigment Cell Melanoma Res.
Source Genre: Journal
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Publ. Info: Oxford : Wiley-Blackwell
Pages: - Volume / Issue: 27 (5) Sequence Number: - Start / End Page: 831 - 834 Identifier: ISSN: 0893-5785
Other: 1600-0749
CoNE: https://pure.mpg.de/cone/journals/resource/pigmentcellres