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  Plasmonic nanopipette biosensor

Masson, F., Breault-Turcot, J., Faid, R., Poirier-Richard, H.-P., Yockell-Lelièvre, H., Lussier, F., et al. (2014). Plasmonic nanopipette biosensor. Analytical Chemistry, 86(18), 8998-9005. doi:10.1021/ac501473c.

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 Creators:
Masson, Francois, Author
Breault-Turcot, Julien, Author
Faid, Rita, Author
Poirier-Richard, Hugo-Pierre, Author
Yockell-Lelièvre, Hélène, Author
Lussier, Félix, Author
Spatz, Joachim P.1, 2, Author           
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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 Abstract: Integrating a SERS immunoassay on a plasmonic "patch clamp" nanopipette enabled nanobiosensing for the detection of IgG. A SERS response was obtained using a sandwich assay benefiting from plasmon coupling between a capture Au nanoparticle (AuNP) on a nanotip and a second AuNP modified with a Raman active reporter and an antibody selective for IgG. The impact of nanoparticle shape and surface coverage was investigated alongside the choice of Raman active reporter, deposition pH, and plasmonic coupling, in an attempt to fully understand the plasmonic properties of nanopipettes and to optimize the nanobiosensor for the detection of IgG. These probes will find applications in various fields due to their nanoscale size leading to the possibility of spatially and temporally addressing their location near cells to monitor secretion of biomolecules.

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Language(s): eng - English
 Dates: 2014-04-152014-08-192014-08-262014-09-16
 Publication Status: Issued
 Pages: 8
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 Table of Contents: -
 Rev. Type: Peer
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Title: Analytical Chemistry
Source Genre: Journal
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Publ. Info: Washington, D.C. : American Chemical Society
Pages: - Volume / Issue: 86 (18) Sequence Number: - Start / End Page: 8998 - 9005 Identifier: Other: 0003-2700
CoNE: https://pure.mpg.de/cone/journals/resource/111032812862552