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  Stable biochemically micro-patterned hydrogel layers control specific cell adhesion and allow long term cyclic tensile strain experiments

Greiner, A. M., Hoffmann, P., Bruellhoff, K., Jungbauer, S., Spatz, J. P., Möller, M., et al. (2014). Stable biochemically micro-patterned hydrogel layers control specific cell adhesion and allow long term cyclic tensile strain experiments. Macromolecular Bioscience, 14(11), 1547-1555. doi:10.1002/mabi.201400261.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-30D5-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-5DA2-1
Genre: Journal Article

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 Creators:
Greiner, Alexandra M., Author
Hoffmann, Peter, Author
Bruellhoff, Kristina, Author
Jungbauer, Simon1, 2, Author              
Spatz, Joachim P.1, 2, Author              
Möller, Martin, Author
Kemkemer, Ralf1, Author              
Groll, Jürgen, Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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Free keywords: hydrogels; mechanical properties; micro-contact printing of fibronectin; specific cell adhesion; spin coating of star polymers; uniaxial cyclic tensile strain
 Abstract: Poly(dimethylsiloxane) can be covalently coated with ultrathin NCO-sP(EO-stat-PO) hydrogel layers which permit covalent binding of cell adhesive moieties, while minimizing unspecific cell adhesion on non-functionalized areas. We applied long term uniaxial cyclic tensile strain (CTS) and revealed (a) the preservation of protein and cell-repellent properties of the NCO-sP(EO-stat-PO) coating and (b) the stability and bioactivity of a covalently bound fibronectin (FN) line pattern. We studied the adhesion of human dermal fibroblast (HDFs) on non-modified NCO-sP(EO-stat-PO) coatings and on the FN. HDFs adhered to FN and oriented their cell bodies and actin fibers along the FN lines independently of the direction of CTS. This mechanical long term stability of the bioactive, patterned surface allows unraveling biomechanical stimuli for cellular signaling and behavior to understand physiological and pathological cell phenomenon. Additionally, it allows for the application in wound healing assays, tissue engineering, and implant development demanding spatial control over specific cell adhesion.

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Language(s): eng - English
 Dates: 2014-05-272014-06-302014-08-062014-11-01
 Publication Status: Published in print
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
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Title: Macromolecular Bioscience
Source Genre: Journal
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Publ. Info: Weinheim, Fed. Rep. of Germany : Wiley-VCH Verlag GmbH
Pages: - Volume / Issue: 14 (11) Sequence Number: - Start / End Page: 1547 - 1555 Identifier: ISSN: 1616-5187
CoNE: https://pure.mpg.de/cone/journals/resource/954925586298_1