English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Switchable adhesive substrates: revealing geometry dependence in collective cell behavior

Rolli, C. G., Nakayama, H., Yamaguchi, K., Spatz, J. P., Kemkemer, R., & Nakanishi, J. (2012). Switchable adhesive substrates: revealing geometry dependence in collective cell behavior. Biomaterials, 33(8), 2409-2418. doi:10.1016/j.biomaterials.2011.12.012.

Item is

Files

show Files
hide Files
:
Biomaterials_33_2012_2409.pdf (Any fulltext), 2MB
 
File Permalink:
-
Name:
Biomaterials_33_2012_2409.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Creators

show
hide
 Creators:
Rolli, Claudio G.1, Author              
Nakayama, Hidekazu, Author
Yamaguchi, Kazuo, Author
Spatz, Joachim P.1, 2, Author              
Kemkemer, Ralf1, Author              
Nakanishi, Jun, Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

Content

show
hide
Free keywords: Collective cell migration; Photopatterning; Wound healing; Cell spreading; Passivation; Cell adhesion
 Abstract: Collective cell migration plays a major role in cancer metastasis and wound healing, therefore, several in vitro assays for studying such behavior have been developed. Using photoswitchable surfaces, we studied collective cell expansion behavior from initially precisely controlled adhesive patterns. A non-adhesive poly(ethylene glycol) (PEG) layer is conjugated to a glass coverslip via 2-nitrobenzyl groups, which cleave upon exposure to UV light, changing the surface from non-cell-adhesive to cell-adhesive without mechanical interference. Initial cell attaching areas are generated in arbitrary shapes via projection exposure through a photomask. After a growth phase, epithelial cell sheets are released from their confinement by a second illumination allowing for collective cell expansion. Our experiments with epithelial cells show that cluster size and boundary curvature modulate the expansion of the cell sheet and the formation of leader cells. At a certain cluster size, characteristics of the expansion behavior change and cells in the core are hardly affected by the boundary release. With donut-like ring structures, we demonstrate a break in symmetry between the behavior of cells along the outer convex boundary and along the inner concave boundary. Additionally, we observe that collective migration characteristics are modulated by the initial incubation time of the cell sheet.

Details

show
hide
Language(s): eng - English
 Dates: 2011-11-222011-12-042011-12-242012-03-01
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Biomaterials
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Guildford, England : Elsevier
Pages: - Volume / Issue: 33 (8) Sequence Number: - Start / End Page: 2409 - 2418 Identifier: ISSN: 0142-9612
CoNE: https://pure.mpg.de/cone/journals/resource/954925472369