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  Effective polyethylene glycol passivation for the inhibition of surface interactions of peripheral blood mononuclear cells and platelets

Sauter, A., Richter, G., Micoulet, A., Martinez, A., Spatz, J. P., & Appel, S. (2013). Effective polyethylene glycol passivation for the inhibition of surface interactions of peripheral blood mononuclear cells and platelets. Biointerphases, 8(1): 14, pp. 1-12. doi:10.1186/1559-4106-8-14.

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Biointerphases_8_2013_8-14.pdf (Any fulltext), 327KB
 
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 Creators:
Sauter, Alexander, Author
Richter, Gunther, Author           
Micoulet, Alexandre1, 2, Author           
Martinez, Aurora, Author
Spatz, Joachim P.1, 2, Author           
Appel, Silke, Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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Free keywords: Polyethylene glycol, Peripheral blood mononuclear cells, Surface passivation, Unspecific interactions
 Abstract: The inhibition of unspecific adhesion of human white blood cells is a prerequisite for applications requiring the control of defined surface interactions. In this study, a passivation agent based on polyethylene glycol (PEG) for glass surfaces was investigated for the use with human peripheral blood mononuclear cells (PBMC). The grafting of 2000 g/mol methoxy-terminated PEG-urea-triethoxysilane (mPEG2000) onto glass surfaces successfully inhibited unspecific spreading of both human PBMC and platelets in all experiments. The prevention of surface interactions was independent on the anticoagulant used during blood collection. The total efficiency to prevent even transient immobilization of PBMC to the PEG modified surfaces was 97 ± 2%. This makes the passivation with PEG a well suited surface modification for preventing unspecific surface interaction in order to study only defined surface interactions of human PBMC.

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Language(s): eng - English
 Dates: 2013-06-052013-06-142013-06-202013-12-01
 Publication Status: Published in print
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Biointerphases
Source Genre: Journal
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Publ. Info: New York, NY : American Vacuum Society
Pages: - Volume / Issue: 8 (1) Sequence Number: 14 Start / End Page: 1 - 12 Identifier: ISSN: 1559-4106
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000220640_1