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  Bioactivity of xerogels as modulators of osteoclastogenesis mediated by connexin 43

Glenske, K., Wagner, A.-S., Hanke, T., Cavalcanti-Adam, E. A., Heinemann, S., Heinemann, C., et al. (2014). Bioactivity of xerogels as modulators of osteoclastogenesis mediated by connexin 43. Biomaterials, 35(5), 1487-1495. doi:10.1016/j.biomaterials.2013.11.002.

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 Creators:
Glenske, Kristina, Author
Wagner, Alena-Svenja, Author
Hanke, Thomas, Author
Cavalcanti-Adam, Elisabetta Ada1, 2, Author           
Heinemann, Sascha, Author
Heinemann, Christiane, Author
Kruppke, Benjamin, Author
Arnhold, Stefan, Author
Moritz, Andreas, Author
Schwab, Elisabeth1, 2, Author           
Worch, Hartmut, Author
Wenisch, Sabine, Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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Free keywords: Bioactivity; Calcium; Osteoclast; Xerogel
 Abstract: In order to investigate the effects of different degrees of bioactivity of xerogels on connexin 43 (cx43) signaling of osteoclasts a cell culture approach was developed. Cells isolated from peripheral blood mononuclear cells were cultured in combination with the xerogels and were harvested for further investigations on day 1, day 5, and day 10. By means of quantitative PCR increased cx43 mRNA levels and coincident decreasing mRNA levels of the calcium sensing receptor, TRAP, and Cathepsin K were detected with increasing bioactivity of the xerogel samples. Additionally, osteoclasts cultured on tissue culture plates were used to perform principle investigations on cell differentiation by means of transmission electron microscopy, life cell imaging, and immunofluorescence, and the results demonstrated that cx43-signaling could be attributed to migration and fusion of osteoclast precursors. Therefore, the positive correlation of cx43 expression with high xerogel bioactivity was caused by proceeding differentiation of the osteoclasts. Finally, the presently observed pattern of cx43 signaling refers to strong effects regarding bioactivity on cx43-associated cell differentiation of osteoclasts influenced by extracellular calcium ions.

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Language(s): eng - English
 Dates: 2013-09-172013-11-012013-11-202014
 Publication Status: Issued
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
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Title: Biomaterials
Source Genre: Journal
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Publ. Info: Guildford, England : Elsevier
Pages: - Volume / Issue: 35 (5) Sequence Number: - Start / End Page: 1487 - 1495 Identifier: ISSN: 0142-9612
CoNE: https://pure.mpg.de/cone/journals/resource/954925472369