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  Interface immobilization chemistry of cRGD-based peptides regulates integrin mediated cell adhesion

Pallarola, D., Bochen, A., Böhm, H., Rechenmacher, F., Sobahi, T., Spatz, J. P., et al. (2014). Interface immobilization chemistry of cRGD-based peptides regulates integrin mediated cell adhesion. Advanced Functional Materials, 24(7), 943-956. doi:10.1002/adfm.201302411.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0015-8CDA-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-6275-6
Genre: Journal Article

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AdvFunctMater_24_2014_943.pdf (Any fulltext), 3MB
 
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 Creators:
Pallarola, Diego1, 2, Author              
Bochen, Alexander, Author
Böhm, Heike1, 2, Author              
Rechenmacher, Florian, Author
Sobahi, Tariq, Author
Spatz, Joachim P.1, 2, Author              
Kessler, Horst, Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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 Abstract: The interaction of specific surface receptors of the integrin family with different extracellular matrix-based ligands is of utmost importance for the cellular adhesion process. A ligand consists of an integrin-binding group, here cyclic RGDfX, a spacer molecule that lifts the integrin-binding group from the surface and a surface anchoring group. c(-RGDfX-) peptides are bound to gold nanoparticle structured surfaces via polyproline, polyethylene glycol or aminohexanoic acid containing spacers of different lengths. Although keeping the integrin-binding c(-RGDfX-) peptides constant for all compounds, changes of the ligand's spacer chemistry and length reveal significant differences in cell adhesion activation and focal adhesion formation. Polyproline-based peptides demonstrate improved cell adhesion kinetics and focal adhesion formation compared with common aminohexanoic acid or polyethylene glycol spacers. Binding activity can additionally be improved by applying ligands with two head groups, inducing a multimeric effect. This study gives insights into spacer-based differences in integrin-driven cell adhesion processes and remarkably highlights the polyproline-based spacers as suitable ligand-presenting templates for surface functionalization.

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Language(s): eng - English
 Dates: 2013-08-282013-07-172013-10-162014-02-19
 Publication Status: Published in print
 Pages: 14
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/adfm.201302411
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Title: Advanced Functional Materials
  Other : Adv. Funct. Mater.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH Verlag GmbH
Pages: - Volume / Issue: 24 (7) Sequence Number: - Start / End Page: 943 - 956 Identifier: ISSN: 1616-301X
CoNE: https://pure.mpg.de/cone/journals/resource/954925596563