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  Combined effects of PEG hydrogel elasticity and cell-adhesive coating on fibroblast adhesion and persistent migration

Missirlis, D., & Spatz, J. P. (2014). Combined effects of PEG hydrogel elasticity and cell-adhesive coating on fibroblast adhesion and persistent migration. Biomacromolecules, 15(1), 195-205. doi:10.1021/bm4014827.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0015-188E-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-6282-8
Genre: Journal Article

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 Creators:
Missirlis, Dimitris1, Author              
Spatz, Joachim P.1, 2, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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 Abstract: The development and use of synthetic, cross-linked, macromolecular substrates with tunable elasticity has been instrumental in revealing the mechanisms by which cells sense and respond to their mechanical microenvironment. We here describe a hydrogel based on radical-free, cross-linked poly(ethylene glycol) to study the effects of both substrate elasticity and type of adhesive coating on fibroblast adhesion and migration. Hydrogel elasticity was controlled through the structure and concentration of branched precursors, which efficiently react via Michael-type addition to produce the polymer network. We found that cell spreading and focal adhesion characteristics are dependent on elasticity for all types of coatings (RGD peptide, fibronectin, vitronectin), albeit with significant differences in magnitude. Importantly, fibroblasts migrated slower but more persistently on stiffer hydrogels, with the effects being more pronounced on fibronectin-coated substrates. Therefore, our results validate the hydrogels presented in this study as suitable for future mechanosensing studies and indicate that cell adhesion, polarity, and associated migration persistence are tuned by substrate elasticity and biochemical properties.

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Language(s): eng - English
 Dates: 2013-11-222013-10-032013-11-252013-12-052014-01-13
 Publication Status: Published in print
 Pages: 11
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 Table of Contents: -
 Rev. Type: Peer
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Title: Biomacromolecules
  Other : Biomacromolecules
Source Genre: Journal
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Publ. Info: Washington, DC : American Chemical Society
Pages: - Volume / Issue: 15 (1) Sequence Number: - Start / End Page: 195 - 205 Identifier: ISSN: 1525-7797
CoNE: https://pure.mpg.de/cone/journals/resource/969870273004