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  Adhesion maturation of neutrophils on nanoscopically presented platelet glycoprotein Ibα

Kruss, S., Erpenbeck, L., Amschler, K., Mundinger, T., Böhm, H., Helms, H.-J., et al. (2013). Adhesion maturation of neutrophils on nanoscopically presented platelet glycoprotein Ibα. ACS Nano, 7(11), 9984-9996. doi:10.1021/nn403923h.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0015-1002-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-77DB-E
Genre: Journal Article

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 Creators:
Kruss, Sebastian1, 2, Author              
Erpenbeck, Luise, Author
Amschler, Katharina, Author
Mundinger, Tabea1, 2, Author              
Böhm, Heike1, 2, Author              
Helms, Hans-Joachim, Author
Friede, Tim, Author
Andrews, Robert, Author
Schön, Michael, Author
Spatz, Joachim P.1, 2, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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Free keywords: biofunctionalization; biomimetic surfaces; cell adhesion; nanopatterning; neutrophils; platelets
 Abstract: Neutrophilic granulocytes play a fundamental role in cardiovascular disease. They interact with platelet aggregates via the integrin Mac-1 and the platelet receptor glycoprotein Ibα (GPIbα). In vivo, GPIbα presentation is highly variable under different physiological and pathophysiological conditions. Here, we quantitatively determined the conditions for neutrophil adhesion in a biomimetic in vitro system, which allowed precise adjustment of the spacings between human GPIbα presented on the nanoscale from 60 to 200 nm. Unlike most conventional nanopatterning approaches, this method provided control over the local receptor density (spacing) rather than just the global receptor density. Under physiological flow conditions, neutrophils required a minimum spacing of GPIbα molecules to successfully adhere. In contrast, under low-flow conditions, neutrophils adhered on all tested spacings with subtle but nonlinear differences in cell response, including spreading area, spreading kinetics, adhesion maturation, and mobility. Surprisingly, Mac-1-dependent neutrophil adhesion was very robust to GPIbα density variations up to 1 order of magnitude. This complex response map indicates that neutrophil adhesion under flow and adhesion maturation are differentially regulated by GPIbα density. Our study reveals how Mac-1/GPIbα interactions govern cell adhesion and how neutrophils process the number of available surface receptors on the nanoscale. In the future, such in vitro studies can be useful to determine optimum therapeutic ranges for targeting this interaction.

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Language(s): eng - English
 Dates: 2013-07-292013-10-012013-10-012013-11-26
 Publication Status: Published in print
 Pages: 13
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 Table of Contents: -
 Rev. Type: Peer
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Title: ACS Nano
Source Genre: Journal
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Publ. Info: Washington, DC : American Chemical Society
Pages: - Volume / Issue: 7 (11) Sequence Number: - Start / End Page: 9984 - 9996 Identifier: Other: 1936-0851
CoNE: https://pure.mpg.de/cone/journals/resource/1936-0851