English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
 Local TagsRelease HistoryDetailsSummary
  Cobalt(III) as a stable and inert mediator ion between NTA and His6- tagged proteins

Wegner, S. V., & Spatz, J. P. (2013). Cobalt(III) as a stable and inert mediator ion between NTA and His6- tagged proteins. Angewandte Chemie International Edition: a journal of the Gesellschaft Deutscher Chemiker, 52(29), 7593-7596. doi:10.1002/anie.201210317.

Item is

 

show all hide all

Basic

show hide
Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0014-C690-D Version Permalink: https://hdl.handle.net/11858/00-001M-0000-002E-7780-7
Genre: Journal Article

Files

show Files
hide Files
:
AngewChemIntEd_52_2013_7593.pdf (Any fulltext), 653KB
 
File Permalink:
-
Name:
AngewChemIntEd_52_2013_7593.pdf
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Locator:
http://onlinelibrary.wiley.com/doi/10.1002/anie.201210317/epdf (Any fulltext)
Description:
-
OA-Status:
Locator:
https://dx.doi.org/10.1002/anie.201210317 (Any fulltext)
Description:
-
OA-Status:

Creators

show
hide
 Creators:
Wegner, Seraphine V.1, 2, Author           
Spatz, Joachim P.1, 2, Author           
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

Content

show
hide
Free keywords: His-tag; cobalt; kinetics; nitrilotriacetic acid; protein modification
 Abstract: Don't let go! The Co(3+)-mediated interaction between nitrilotriacetic acid (NTA) and the His6-tag is so stable and inert towards ligand exchange that it has a half-life of 7 days in the presence of imidazole and survives even under strongly chelating as well as reducing conditions, unlike the commonly used Ni(2+) or Co(2+) complexes. Possible applications include the separation of labeled proteins and the stable immobilization of proteins on surfaces.

Details

show
hide
Language(s): eng - English
 Dates: 2013-03-072012-12-272013-06-052013-06-052013-07-15
 Publication Status: Issued
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/anie.201210317
URI: https://www.ncbi.nlm.nih.gov/pubmed/23740821
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Angewandte Chemie International Edition : a journal of the Gesellschaft Deutscher Chemiker
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Weinheim/Bergstr. : Verlag Chemie
Pages: - Volume / Issue: 52 (29) Sequence Number: - Start / End Page: 7593 - 7596 Identifier: ISSN: 0570-0833
CoNE: https://pure.mpg.de/cone/journals/resource/110984073528720