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  Cobalt(III) as a stable and inert mediator ion between NTA and His6- tagged proteins

Wegner, S. V., & Spatz, J. P. (2013). Cobalt(III) as a stable and inert mediator ion between NTA and His6- tagged proteins. Angewandte Chemie International Edition: a journal of the Gesellschaft Deutscher Chemiker, 52(29), 7593-7596. doi:10.1002/anie.201210317.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-C690-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-7780-7
Genre: Journal Article

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AngewChemIntEd_52_2013_7593.pdf (Any fulltext), 653KB
 
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 Creators:
Wegner, Seraphine V.1, 2, Author              
Spatz, Joachim P.1, 2, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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Free keywords: His-tag; cobalt; kinetics; nitrilotriacetic acid; protein modification
 Abstract: Don't let go! The Co(3+)-mediated interaction between nitrilotriacetic acid (NTA) and the His6-tag is so stable and inert towards ligand exchange that it has a half-life of 7 days in the presence of imidazole and survives even under strongly chelating as well as reducing conditions, unlike the commonly used Ni(2+) or Co(2+) complexes. Possible applications include the separation of labeled proteins and the stable immobilization of proteins on surfaces.

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Language(s): eng - English
 Dates: 2013-03-072012-12-272013-06-052013-06-052013-07-15
 Publication Status: Published in print
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
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Title: Angewandte Chemie International Edition : a journal of the Gesellschaft Deutscher Chemiker
Source Genre: Journal
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Publ. Info: Weinheim/Bergstr. : Verlag Chemie
Pages: - Volume / Issue: 52 (29) Sequence Number: - Start / End Page: 7593 - 7596 Identifier: ISSN: 0570-0833
CoNE: https://pure.mpg.de/cone/journals/resource/110984073528720