English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Soft micropillar interfaces of distinct biomechanics govern behaviour of periodontal cells

Mussig, E., Schulz, S., Spatz, J. P., Ziegler, N., Tomakidi, P., & Steinberg, T. (2010). Soft micropillar interfaces of distinct biomechanics govern behaviour of periodontal cells. European Journal of Cell Biology: EJCB, 89(4), 315-325. doi:10.1016/j.ejcb.2009.08.004.

Item is

Files

show Files
hide Files
:
EurJCellBiol_89_2010_315.pdf (Any fulltext), 2MB
 
File Permalink:
-
Name:
EurJCellBiol_89_2010_315.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Creators

show
hide
 Creators:
Mussig, Eva, Author
Schulz, Simon1, Author              
Spatz, Joachim P.1, 2, Author              
Ziegler, Nelli, Author
Tomakidi, Pascal, Author
Steinberg, Thorsten, Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

Content

show
hide
Free keywords: biofunctionalisation; biomechanics; periodontium; elasticity; polydimethylsiloxane (PDMS); micropillar interfaces
 Abstract: A soft micropillar extracellular environment of distinct biomechanics is established by fabricating polydimethylsiloxane (PDMS) interfaces with pillar distances of 5, 7, 9 and 11 microm and elasticity moduli of 0.6, 1.0 and 3.5 Mega Pascal. To allow for cell adhesion, the biomimetic concept of pillar head fibronectin (FN) biofunctionalisation is applied. This environmental set-up aims at the analysis of favourable conditions for cell behaviour of three periodontal cell-types, here reflected by the establishment of regular cell morphology and optimal collagen gene expression. Biomechanics of these predefined functionalized model surfaces reveal progressive deterioration of regular cell morphology with increasing pillar distance, independent from pillar elasticity and cell type. Analysis of collagen gene expression demonstrates interdependency to the elasticity and the micropattern of the extracellular environment in all cell types under study. The results suggest that biomechanics of the extracellular environment govern tissue-specific cell behaviour in different periodontal cell types. Moreover, they form the basis for the creation of new biomaterials which address distinct cell functions by specific biomechanical properties.

Details

show
hide
Language(s): eng - English
 Dates: 2009-08-272009-07-062009-08-272009-09-252010-04-01
 Publication Status: Published in print
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: European Journal of Cell Biology : EJCB
  Other : Eur. J. Cell Biol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Stuttgart : Wissenschaftliche Verlagsgesellschaft.
Pages: - Volume / Issue: 89 (4) Sequence Number: - Start / End Page: 315 - 325 Identifier: ISSN: 0070-2463
CoNE: https://pure.mpg.de/cone/journals/resource/954925486755