English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Two characteristic regimes in frequency dependent dynamic reorientation of fibroblasts on cyclically stretched substrates

Jungbauer, S., Gao, H., Spatz, J. P., & Kemkemer, R. (2008). Two characteristic regimes in frequency dependent dynamic reorientation of fibroblasts on cyclically stretched substrates. Biophysical Journal, 95(7), 3470-3478. doi:10.1529/biophysj.107.128611.

Item is

Files

show Files
hide Files
:
BiophysJ_95_2008_3470.pdf (Any fulltext), 606KB
 
File Permalink:
-
Name:
BiophysJ_95_2008_3470.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Creators

show
hide
 Creators:
Jungbauer, Simon1, 2, Author              
Gao, Huajian, Author
Spatz, Joachim P.1, 2, Author              
Kemkemer, Ralf1, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

Content

show
hide
Free keywords: -
 Abstract: Cells adherent on a cyclically stretched substrate with a periodically varying uniaxial strain are known to dynamically reorient nearly perpendicular to the strain direction. We investigate the dynamic reorientation of rat embryonic and human fibroblast cells over a range of stretching frequency from 0.0001 to 20 s(-1) and strain amplitude from 1% to 15%. We report quantitative measurements that show that the mean cell orientation changes exponentially with a frequency-dependent characteristic time from 1 to 5 h. At subconfluent cell densities, this characteristic time for reorientation shows two characteristic regimes as a function of frequency. For frequencies below 1 s(-1), the characteristic time decreases with a power law as the frequency increases. For frequencies above 1 s(-1), it saturates at a constant value. In addition, a minimum threshold frequency is found below that no significant cell reorientation occurs. Our results are consistent for the two different fibroblast types and indicate a saturation of molecular mechanisms of mechanotransduction or response machinery for subconfluent cells within the frequency regime under investigation. For confluent cell layers, we observe similar behaviors of reorientation under cyclic stretch but no saturation in the characteristic time with frequency, suggesting that cell-cell contacts can play an important role in the response machinery of cells under mechanical strain.

Details

show
hide
Language(s): eng - English
 Dates: 2008-01-022008-05-022008-05-302008-10-01
 Publication Status: Published in print
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Biophysical Journal
  Other : Biophys. J.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 95 (7) Sequence Number: - Start / End Page: 3470 - 3478 Identifier: Other: 0006-3495
CoNE: https://pure.mpg.de/cone/journals/resource/954925385117