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  Impact of tumor cell cytoskeleton organization on invasiveness and migration: a microchannel-based approach

Rolli, C. G., Seufferlein, T., Kemkemer, R., & Spatz, J. P. (2010). Impact of tumor cell cytoskeleton organization on invasiveness and migration: a microchannel-based approach. PLoS One, 5(1): e8726, pp. 1-8. doi:10.1371/journal.pone.0008726.

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Rolli, Claudio Gavino1, Author              
Seufferlein, Thomas, Author
Kemkemer, Ralf1, Author              
Spatz, Joachim P.1, 2, Author              
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1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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 Abstract: Cell migration is a fundamental feature of the interaction of cells with their surrounding. The cell's stiffness and ability to deform itself are two major characteristics that rule migration behavior especially in three-dimensional tissue. We simulate this situation making use of a micro-fabricated migration chip to test the active invasive behavior of pancreatic cancer cells (Panc-1) into narrow channels. At a channel width of 7 microm cell migration through the channels was significantly impeded due to size exclusion. A striking increase in cell invasiveness was observed once the cells were treated with the bioactive lipid sphingosylphosphorylcholine (SPC) that leads to a reorganization of the cell's keratin network, an enhancement of the cell's deformability, and also an increase in the cell's migration speed on flat surfaces. The migration speed of the highly deformed cells inside the channels was three times higher than of cells on flat substrates but was not affected upon SPC treatment. Cells inside the channels migrated predominantly by smooth sliding while maintaining constant cell length. In contrast, cells on adhesion mediating narrow lines moved in a stepwise way, characterized by fluctuations in cell length. Taken together, with our migration chip we demonstrate that the dimensionality of the environment strongly affects the migration phenotype and we suggest that the spatial cytoskeletal keratin organization correlates with the tumor cell's invasive potential.

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Language(s): eng - English
 Dates: 2009-10-232009-12-212010-01-15
 Publication Status: Published online
 Pages: 8
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 Table of Contents: -
 Rev. Type: Peer
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Title: PLoS One
Source Genre: Journal
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Publ. Info: San Francisco, CA : Public Library of Science
Pages: - Volume / Issue: 5 (1) Sequence Number: e8726 Start / End Page: 1 - 8 Identifier: ISSN: 1932-6203
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000277850