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  Optical force sensor array in a microfluidic device based on holographic optical tweezers

Uhrig, K., Kurre, R., Schmitz, C., Curtis, J. E., Haraszti, T., Clemen, A.-E.-M., et al. (2009). Optical force sensor array in a microfluidic device based on holographic optical tweezers. Lab on a Chip, 9(5), 661-668. doi:10.1039/b817633g.

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 Creators:
Uhrig, Kai1, Author              
Kurre, Rainer, Author
Schmitz, Christian, Author
Curtis, Jennifer E.1, Author              
Haraszti, Tamás1, 2, Author              
Clemen, Anabel E.-M.1, Author              
Spatz, Joachim P.1, 2, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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Free keywords: MPI für Intelligente Systeme; Abt. Spatz;
 Abstract: Holographic optical tweezers (HOT) are a versatile technology, with which complex arrays and movements of optical traps can be realized to manipulate multiple microparticles in parallel and to measure the forces affecting them in the piconewton range. We report on the combination of HOT with a fluorescence microscope and a stop-flow, multi-channel microfluidic device. The integration of a high-speed camera into the setup allows for the calibration of all the traps simultaneously both using Boltzmann statistics or the power spectrum density of the particle diffusion within the optical traps. This setup permits complete spatial, chemical and visual control of the microenvironment applicable to probing chemo-mechanical properties of cellular or subcellular structures. As an example we constructed a biomimetic, quasi-two-dimensional actin network on an array of trapped polystyrene microspheres inside the microfluidic chamber. During crosslinking of the actin filaments by Mg(2+) ions, we observe the build up of mechanical tension throughout the actin network. Thus, we demonstrate how our integrated HOT-microfluidics platform can be used as a reconfigurable force sensor array with piconewton resolution to investigate chemo-mechanical processes.

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Language(s): eng - English
 Dates: 2008-10-072009-01-052009-01-232009-03-07
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Lab on a Chip
  Other : Lab Chip
Source Genre: Journal
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Publ. Info: Cambridge : Royal Society of Chemistry
Pages: - Volume / Issue: 9 (5) Sequence Number: - Start / End Page: 661 - 668 Identifier: Other: 1473-0197
CoNE: https://pure.mpg.de/cone/journals/resource/14730197