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  Biomimetic models of the actin cortex

Haraszti, T., Schulz, S., Uhrig, K., Kurre, R., Roos, W., Schmitz, C. H. J., et al. (2009). Biomimetic models of the actin cortex. Biophysical Reviews and Letters, 4(1&2), 17-32. doi:10.1142/S1793048009001009.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-3DEE-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-7BDF-4
Genre: Journal Article

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BiophysRevLett_4_2009_17.pdf (Any fulltext), 3MB
 
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 Creators:
Haraszti, Tamas1, 2, Author              
Schulz, Simon1, Author              
Uhrig, Kai1, Author              
Kurre, Rainer, Author
Roos, Wouter1, Author              
Schmitz, Christian H. J., Author
Curtis, Jennifer E.1, Author              
Maier, Timo1, 2, Author              
Clemen, Anabel E.-M.1, Author              
Spatz, Joachim P.1, 2, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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Free keywords: Microfluidics; actin; cortex model; crosslinking; filamin; alpha-actinin; micropillar; holographic optical tweezer
 Abstract: The cytoskeleton is an actively regulated complex network in the cell. One of the most researched components is actin. In our work we developed and tested two microfluidic systems both being applicable to construct quasi 2-dimensional biomimetic actin networks. The first system uses polydimethylsiloxane micropillars, the other polystyrene microparticles held by holographic optical tweezers as anchoring points. Our devices provide actin networks with mesh sizes from a few micrometers up to the order of 10 micrometers. Qualitative analysis shows similar network formation in both systems. Crosslinking was tested using filamin, α-actinin, Ca and Mg ions. The crosslinking process is characterized by a zipping like event, which is limited only by the high stretching modulus of the actin filaments.

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Language(s): eng - English
 Dates: 2009-04-01
 Publication Status: Published in print
 Pages: 16
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 432982
DOI: 10.1142/S1793048009001009
 Degree: -

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Title: Biophysical Reviews and Letters
Source Genre: Journal
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Publ. Info: Singapore : World Scientific
Pages: - Volume / Issue: 4 (1&2) Sequence Number: - Start / End Page: 17 - 32 Identifier: ISSN: 1793-0480
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000220640