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  Spectral analysis by XANES reveals that GPNMB influences the chemical composition of intact melanosomes

Haraszti, T., Trantow, C. M., Hedberg-Buenz, A., Grunze, M., & Anderson, M. G. (2011). Spectral analysis by XANES reveals that GPNMB influences the chemical composition of intact melanosomes. Pigment Cell & Melanoma Research, 24(1), 187-196. doi:10.1111/j.1755-148X.2010.00788.x.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-4D53-5 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-9357-7
Genre: Journal Article

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PigmentCellMelanomaRes_24_2011_187.pdf (Any fulltext), 681KB
 
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 Creators:
Haraszti, Tamas1, 2, Author              
Trantow, Colleen M., Author
Hedberg-Buenz, Adam, Author
Grunze, Michael1, Author              
Anderson, Michael G., Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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Free keywords: genetic mapping; melanin; melanocytes; melanosomes; X-ray absorption near-edge structure analysis spectroscopy
 Abstract: GPNMB is a unique melanosomal protein. Unlike many melanosomal proteins, GPNMB has not been associated with any forms of albinism, and it is unclear whether GPNMB has any direct influence on melanosomes. Here, melanosomes from congenic strains of C57BL/6J mice mutant for Gpnmb are compared to strain-matched controls using standard transmission electron microscopy and synchrotron-based X-ray absorption near-edge structure analysis (XANES). Whereas electron microscopy did not detect any ultrastructural changes in melanosomes lacking functional GPNMB, XANES uncovered multiple spectral phenotypes. These results directly demonstrate that GPNMB influences the chemical composition of melanosomes and more broadly illustrate the potential for using genetic approaches in combination with nano-imaging technologies to study organelle biology.

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Language(s): eng - English
 Dates: 2010-09-292010-04-082010-09-292010-10-042011-02-01
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Pigment Cell & Melanoma Research
  Other : (Formerly: Pigment Cell Research)
  Abbreviation : Pigment Cell Melanoma Res.
Source Genre: Journal
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Publ. Info: Oxford : Wiley-Blackwell
Pages: - Volume / Issue: 24 (1) Sequence Number: - Start / End Page: 187 - 196 Identifier: ISSN: 0893-5785
Other: 1600-0749
CoNE: https://pure.mpg.de/cone/journals/resource/pigmentcellres