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  GIT1 phosphorylation on serine 46 by PKD3 regulates paxillin trafficking and cellular protrusive activity

Huck, B., Kemkemer, R., Franz-Wachtel, M., Macek, B., Hausser, A., & Olayioye, M. A. (2012). GIT1 phosphorylation on serine 46 by PKD3 regulates paxillin trafficking and cellular protrusive activity. The Journal of Biological Chemistry, 287(41), 34604-34613. doi:10.1074/jbc.M112.374652.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-AAFD-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-5F25-D
Genre: Journal Article

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JBiolChem_287_2012_34604.pdf (Any fulltext), 2MB
 
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 Creators:
Huck, Bettina, Author
Kemkemer, Ralf1, Author              
Franz-Wachtel, Mirita, Author
Macek, Boris, Author
Hausser, Angelika, Author
Olayioye, Monilola A., Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

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 Abstract: The continuous assembly and disassembly of focal adhesions is required for efficient cell spreading and migration. The G-protein-coupled receptor kinase-interacting protein 1 (GIT1) is a multidomain protein whose dynamic localization to sites of cytoskeletal remodeling is critically involved in the regulation of these processes. Here we provide evidence that the subcellular localization of GIT1 is regulated by protein kinase D3 (PKD3) through direct phosphorylation on serine 46. GIT1 phosphorylation on serine 46 was abrograted by PKD3 depletion, thereby identifying GIT1 as the first specific substrate for this kinase. A GIT1 S46D phosphomimetic mutant localized to motile, paxillin-positive cytoplasmic complexes, whereas the phosphorylation-deficient GIT1 S46A was enriched in focal adhesions. We propose that phosphorylation of GIT1 on serine 46 by PKD3 represents a molecular switch by which GIT1 localization, paxillin trafficking, and cellular protrusive activity are regulated.

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Language(s): eng - English
 Dates: 2012-07-272012-04-242012-08-132012-10-05
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
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Title: The Journal of Biological Chemistry
  Other : JBC
Source Genre: Journal
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Publ. Info: Baltimore, etc. : American Society for Biochemistry and Molecular Biology [etc.]
Pages: - Volume / Issue: 287 (41) Sequence Number: - Start / End Page: 34604 - 34613 Identifier: ISSN: 0021-9258
CoNE: https://pure.mpg.de/cone/journals/resource/954925410826_1