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  Single cell force spectroscopy of T cells recognizing a myelin-derived peptide on antigen presenting cells

Hoffmann, S., Hosseini, B. H., Hecker, M., Louban, I., Bulbuc, N., Garbi, N., et al. (2011). Single cell force spectroscopy of T cells recognizing a myelin-derived peptide on antigen presenting cells. Immunology Letters, 136(1), 13-20. doi:10.1016/j.imlet.2010.11.005.

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ImmunolLett_136_2011_13.pdf (Any fulltext), 923KB
 
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 Creators:
Hoffmann, Sabrina1, Author              
Hosseini, Babak H.1, Author              
Hecker, Markus, Author
Louban, Ilia1, Author              
Bulbuc, Nadja, Author
Garbi, Natalio, Author
Wabnitz, Guido H., Author
Samstag, Yvonne, Author
Spatz, Joachim P.1, 2, Author              
Hämmerling, Günter J., Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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Free keywords: Antigen presentation, atomic force microscopy, cellular interaction forces, intercellular adhesion molecule-1 (ICAM-1)
 Abstract: T-cell recognition of peptide-MHC complexes on APCs requires cell-cell interactions. The molecular events leading to T-cell activation have been extensively investigated, but the underlying physical binding forces between T-cells and APCs are largely unknown. We used single cell force spectroscopy for quantitation of interaction forces between T-cells and APCs presenting a tolerogenic peptide derived from myelin basic protein. When T-cells were brought into contact with peptide-loaded APCs, interaction forces increased with time from about 0.5nN after 10s interaction to about 15nN after 30min. In the absence of antigen, or when ICAM-1-negative APC was used, no increase in binding forces was observed. The temporal development of interaction forces correlated with the kinetics of immune synapse formation, as determined by LFA-1 and TCR enrichment at the interface of T-cell/APC conjugates using high throughput multispectral imaging flow cytometry. Together, these results suggest that ICAM-1/LFA-1 redistribution to the contact area is mainly responsible for development of strong interaction forces. High forces will keep T-cells and APCs in tight contact, thereby providing a platform for optimal interaction between TCRs and peptide-MHC complexes.

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Language(s): eng - English
 Dates: 2010-11-082010-10-082010-11-152010-11-262011-04-30
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Immunology Letters
  Other : Immunol. Lett.
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 136 (1) Sequence Number: - Start / End Page: 13 - 20 Identifier: ISSN: 0165-2478
CoNE: https://pure.mpg.de/cone/journals/resource/954925481610