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  Impact of substrate elasticity on human hematopoietic stem and progenitor cell adhesion and motility

Lee-Thedieck, C., Rauch, N., Fiammengo, R., Klein, G., & Spatz, J. P. (2012). Impact of substrate elasticity on human hematopoietic stem and progenitor cell adhesion and motility. Journal of Cell Science, 125(16), 3765-3775. doi:10.1242/jcs.095596.

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 Creators:
Lee-Thedieck, Cornelia1, Author              
Rauch, Nicole1, Author              
Fiammengo, Roberto1, Author              
Klein, Gerd, Author
Spatz, Joachim P.1, 2, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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Free keywords: Hematopoietic stem and progenitor cells, Mechanotransduction, Elasticity, Hematopoietic stem cell niche
 Abstract: In the bone marrow, hematopoietic stem cells (HSCs) reside in endosteal and vascular niches. The interactions with the niches are essential for the maintenance of HSC number and properties. Although the molecular nature of these interactions is well understood, little is known about the role of physical parameters such as matrix elasticity. Osteoblasts, the major cellular component of the endosteal HSC niche, flatten during HSC mobilization. We show that this process is accompanied by osteoblast stiffening, demonstrating that not only biochemical signals but also mechanical properties of the niche are modulated. HSCs react to stiffer substrates with increased cell adhesion and migration, which could facilitate the exit of HSCs from the niche. These results indicate that matrix elasticity is an important factor in regulating the retention of HSCs in the endosteal niche and should be considered in attempts to propagate HSCs in vitro for clinical applications.

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Language(s): eng - English
 Dates: 2012-04-102012-05-022012-08-15
 Publication Status: Published in print
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Journal of Cell Science
Source Genre: Journal
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Publ. Info: Cambridge, U.K. : Co. of Biologists
Pages: - Volume / Issue: 125 (16) Sequence Number: - Start / End Page: 3765 - 3775 Identifier: ISSN: 0021-9533
CoNE: https://pure.mpg.de/cone/journals/resource/954925326678