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  Direct manipulation of malaria parasites with optical tweezers reveals distinct functions of plasmodium surface proteins

Hegge, S., Uhrig, K., Streichfuss, M., Kynast-Wolf, G., Matuschewski, K., Spatz, J. P., et al. (2012). Direct manipulation of malaria parasites with optical tweezers reveals distinct functions of plasmodium surface proteins. ACS Nano, 6(6), 4648-4662. doi:10.1021/nn203616u.

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Datensatz-Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-4B90-B Versions-Permalink: https://hdl.handle.net/11858/00-001M-0000-002E-7C23-2
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http://pubs.acs.org/doi/pdf/10.1021/nn203616u (beliebiger Volltext)
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 Urheber:
Hegge, Stephan, Autor
Uhrig, Kai1, Autor           
Streichfuss, Martin1, 2, Autor           
Kynast-Wolf, Gisela, Autor
Matuschewski, Kai, Autor
Spatz, Joachim P.1, 2, Autor           
Frischknecht, Friedrich, Autor
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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Schlagwörter: optical traps . apicomplexa . sporozoites . gliding motility . adhesin . cohesion
 Zusammenfassung: Plasmodium sporozoite motility is essential for establishing malaria infections. It depends on initial adhesion to a substrate as well as the continuous turnover of discrete adhesion sites. Adhesion and motility are mediated by a dynamic actin cytoskeleton and surface proteins. The mode of adhesion formation and the integration of adhesion forces into fast and continuous forward locomotion remain largely unknown. Here, we use optical tweezers to directly trap individual parasites and probe adhesion formation. We find that sporozoites lacking the surface proteins TRAP and S6 display distinct defects in initial adhesion; trap(-) sporozoites adhere preferentially with their front end, while s6(-) sporozoites show no such preference. The cohesive strength of the initial adhesion site is differently affected by actin filament depolymerization at distinct adhesion sites along the parasite for trap(-) and s6(-) sporozoites. These spatial differences between TRAP and S6 in their functional interaction with actin filaments show that these proteins have nonredundant roles during adhesion and motility. We suggest that complex protein–protein interactions and signaling events govern the regulation of parasite gliding at different sites along the parasite. Investigating how these events are coordinated will be essential for our understanding of sporozoite gliding motility, which is crucial for malaria infection. Laser tweezers will be a valuable part of the toolset.

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Sprache(n): eng - English
 Datum: 2011-09-202012-05-082012-05-082012
 Publikationsstatus: Erschienen
 Seiten: 15
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: eDoc: 611338
DOI: 10.1021/nn203616u
 Art des Abschluß: -

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Titel: ACS Nano
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Washington, DC : American Chemical Society
Seiten: - Band / Heft: 6 (6) Artikelnummer: - Start- / Endseite: 4648 - 4662 Identifikator: ISSN: 1936-0851
CoNE: https://pure.mpg.de/cone/journals/resource/1936-0851