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  Microtubule gliding and crosslinked microtubule networks on micro-pillar interfaces

Roos, W., Ulmer, J., Gräter, S., Surrey, T., & Spatz, J. P. (2005). Microtubule gliding and crosslinked microtubule networks on micro-pillar interfaces. Nano Letters, 5(12), 2630-2634. doi:10.1021/nl051865j.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-493E-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-7C5A-6
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NanoLett_5_2005_2630.pdf (Any fulltext), 364KB
 
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 Creators:
Roos, Wouter1, Author              
Ulmer, Jens1, Author              
Gräter, Stefan1, Author              
Surrey, Thomas, Author
Spatz, Joachim P.1, 2, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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 Abstract: We combined biochemical and topographical patterning to achieve motor-driven microtubule gliding on top of microfabricated pillar arrays with limited and controllable surface interactions of gliding microtubules. Kinesins immobilized on pillar heads pushed microtubules from the top of one micropillar to the next bridging up to 20 μm deep gaps filled with buffer solution. Distances of more than 10 μm were by-passed, and microtubule buckling was occasionally observed. The velocity distributions of microtubules gliding on poly(dimethylsiloxane) (PDMS) pillars, on flat PDMS, and on glass were found to be different, most likely due to topological and/or chemical differences between the substrates. We also used pillar arrays to suspend cross-linked microtubule networks, whose structural characteristics were governed by the topographical characteristics of the pillar pattern. These experiments open new possibilities to study the dynamics and the self-organization of motor/microtubule networks in defined topologically structured environments.

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Language(s): eng - English
 Dates: 2005-10-212005-09-182005-11-122005-12-01
 Publication Status: Published in print
 Pages: 5
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 Rev. Type: Peer
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Title: Nano Letters
  Abbreviation : Nano Lett.
Source Genre: Journal
 Creator(s):
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Publ. Info: Washington, DC : American Chemical Society
Pages: - Volume / Issue: 5 (12) Sequence Number: - Start / End Page: 2630 - 2634 Identifier: ISSN: 1530-6984
CoNE: https://pure.mpg.de/cone/journals/resource/110978984570403