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  Live cell adhesion assay with attenuated total reflection infrared spectroscopy

Schmidt, M., Wolfram, T., Rumpler, M., Tripp, C. P., & Grunze, M. (2007). Live cell adhesion assay with attenuated total reflection infrared spectroscopy. Biointerphases, 2(1), 1-5. doi:10.1116/1.2710336.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-44D3-7 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-002E-6A69-6
Genre: Journal Article

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Biointerphases_2_2007_1.pdf (Any fulltext), 674KB
 
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http://link.springer.com/content/pdf/10.1116%2F1.2710336.pdf (Any fulltext)
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https://dx.doi.org/10.1116/1.2710336 (Any fulltext)
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 Creators:
Schmidt, Martin, Author
Wolfram, Tobias, Author
Rumpler, Monika, Author
Tripp, Carl P., Author
Grunze, Michael1, Author           
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

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 Abstract: Living confluent fish fibroblast cells RTG-P1 from rainbow trout adherent on diamond were examined by attenuated total reflection (ATR) infrared (IR) spectroscopy. In particular, IR spectra were recorded dynamically during the adsorption of the cells onto the diamond and during their biochemically induced structural responses to the subsequent addition of trypsin and cytochalasin D. It is shown that changes in the IR spectra result from changes in cell morphology and surface coverage. The results demonstrate the potential and the applicability of ATR IR spectroscopy for live cell adhesion assays.

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Language(s): eng - English
 Dates: 2007
 Publication Status: Issued
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 366657
DOI: 10.1116/1.2710336
URI: https://www.ncbi.nlm.nih.gov/pubmed/20408630
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Title: Biointerphases
Source Genre: Journal
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Publ. Info: New York, NY : American Vacuum Society
Pages: - Volume / Issue: 2 (1) Sequence Number: - Start / End Page: 1 - 5 Identifier: ISSN: 1559-4106
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000220640_1