English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Protein repellent properties of covalently attached PEG coatings on nanostructured SiO2 based interfaces

Blümmel, J., Perschmann, N., Aydin, D., Drinjakovic, J., Surrey, T., Lopez-Garcia, M., et al. (2007). Protein repellent properties of covalently attached PEG coatings on nanostructured SiO2 based interfaces. Biomaterials, 28(32), 4739-4747. doi:10.1016/j.biomaterials.2007.07.038.

Item is

Files

show Files
hide Files
:
Biomaterials_28_2007_4739.pdf (Any fulltext), 689KB
 
File Permalink:
-
Name:
Biomaterials_28_2007_4739.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Creators

show
hide
 Creators:
Blümmel, Jacques1, Author              
Perschmann, Nadine1, 2, Author              
Aydin, Daniel1, Author              
Drinjakovic, Jovana, Author
Surrey, Thomas, Author
Lopez-Garcia, Monica, Author
Kessler, Horst, Author
Spatz, Joachim P.1, 2, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

Content

show
hide
Free keywords: Poly(ethylene glycol) (PEG); Cell adhesion; Protein adsorption; Quartz crystal microbalance (QCM); Nanostructures; Biofunctionalization
 Abstract: In this study, we report the systematic comparison of different poly(ethylene glycol) (PEG) self-assembled monolayers on glass with respect to their protein adsorption and cell adhesion resistance. Combining PEGylation with micellar nanolithography allowed the formation of gold nanoparticle arrays on glass and selective coverage of the free glass area by PEG. The gold nanoparticles serve as anchor points for the attachment of individual proteins and peptides such as the cell-matrix adhesion promoting cyclic RGDfK motif or the kinesin motor protein Eg5. The capability of the motor protein to bind microtubules remained unaffected by the immobilization. It was shown that the film thickness of a water swollen PEG layer is crucial to maximize the interaction between proteins and peptides with the nanostructures. Non-specific interaction between cells or microtubules and the surface was minimized. The optimum PEG layer thickness correlated with the size of gold nanoparticles which was approximately 5 nm.

Details

show
hide
Language(s): eng - English
 Dates: 2007-04-222007-07-152007-08-132007-11-01
 Publication Status: Published in print
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Biomaterials
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Guildford, England : Elsevier
Pages: - Volume / Issue: 28 (32) Sequence Number: - Start / End Page: 4739 - 4747 Identifier: ISSN: 0142-9612
CoNE: https://pure.mpg.de/cone/journals/resource/954925472369