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  Site-specific presentation of single recombinant proteins in defined nanoarrays

Wolfram, T., Belz, F., Schoen, T., & Spatz, J. P. (2007). Site-specific presentation of single recombinant proteins in defined nanoarrays. Biointerphases, 2(1), 44-48. doi:10.1116/1.2713991.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-4349-7 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-002E-7C66-A
Genre: Journal Article

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Biointerphases_2_2007_44.pdf (Any fulltext), 706KB
 
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http://avs.scitation.org/doi/pdf/10.1116/1.2713991 (Any fulltext)
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https://dx.doi.org/10.1116/1.2713991 (Any fulltext)
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 Creators:
Wolfram, Tobias, Author
Belz, Ferdinand1, Author           
Schoen, Tobias, Author
Spatz, Joachim P.1, 2, Author           
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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 Abstract: The authors describe the deposition of single biomolecules on substrates at defined spacing by pure self-assembly. The substrate is equipped with an array of 8 nm large gold particles which form the template for biomolecule binding. The authors verified the successful binding of single biomolecules via specific antibody labeling and imaging by fluorescence microscopy. Scanning force microscopy provided evidence that every gold nanoparticle of the pattern is occupied by at least one biomolecule. Furthermore, gold conjugated secondary antibodies in combination with scanning electron microscopy proved that at least 75% of the nanoparticles carried only one active biomolecule. The precision given by such surface densities is molecularly defined and such considerably higher than in any other case reported so far.

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Language(s): eng - English
 Dates: 2006-12-222007-02-092007-03-292007-03-29
 Publication Status: Issued
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 313025
DOI: 10.1116/1.2713991
URI: https://www.ncbi.nlm.nih.gov/pubmed/20408635
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Title: Biointerphases
Source Genre: Journal
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Publ. Info: New York, NY : American Vacuum Society
Pages: - Volume / Issue: 2 (1) Sequence Number: - Start / End Page: 44 - 48 Identifier: ISSN: 1559-4106
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000220640_1