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  Connective-tissue fibroblasts established on micropillar interfaces are pivotal for epithelial tissue morphogenesis

Mussig, E., Steinberg, T., Schulz, S., Spatz, J. P., Ulmer, J., Grabe, N., et al. (2008). Connective-tissue fibroblasts established on micropillar interfaces are pivotal for epithelial tissue morphogenesis. Advanced Functional Materials, 18(19), 2919-2929. doi:10.1002/adfm.200800381.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-414E-2 Version Permalink: http://hdl.handle.net/21.11116/0000-0000-E339-0
Genre: Journal Article

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AdvFunctMater_18_2008_2919.pdf (Any fulltext), 1000KB
 
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 Creators:
Mussig, Eva, Author
Steinberg, Thorsten, Author
Schulz, Simon1, Author              
Spatz, Joachim P.1, 2, Author              
Ulmer, Jens1, Author              
Grabe, Niels, Author
Kohl, Annette, Author
Komposch, Gerda, Author
Tomakidi, Pascal, Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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 Abstract: Polydimethylsiloxane (PDMS) pillar arrays are applied as a biomechanical microenvironment to establish gingival connective-tissue fibroblasts (GCTFs) and to further analyze the pivotal role of GCTFs in epithelial-tissue morphogenesis. GCTFs are known to exert successful adhesion and growth on fibronectin immobilized on pillar heads, over time, concomitant with the increased gene expression of vimentin and collagen type-I. GCTF-populated pillar arrays clearly reveal that epithelial-tissue morphogenesis of immortalized human gingival keratinocytes (IHGKs), co-cultured for 7 and 14 days, parallels the in vivo phenotype more closely, when compared with GCTF-free control arrays. This in vivo-like phenotype is substantiated by higher mRNA levels for keratin 1, involucrin and filaggrin differentiation markers. Furthermore, it is reflected by a tissue-specific protein orientation of the aforementioned molecules, and also of the cell-to-cell contact forming desmoplakin and the basement membrane constituents, laminin-5, laminin-1/10, and collagen type-IV. These experiments suggest that the in vivo-like phenotype of the IHGK is governed by the GCTFs growing on the micropillar interfaces. Moreover, they form the basis for the optimization or neogeneration of biomaterials by varying predefined microenvironmetal parameters to achieve an in vivo-like cell growth and differentiation, indispensable for tissue morphogenesis during regeneration.

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Language(s): eng - English
 Dates: 2008-03-212008-03-172008-09-222008-09-222008-10-09
 Publication Status: Published in print
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/adfm.200800381
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Title: Advanced Functional Materials
  Other : Adv. Funct. Mater.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH Verlag GmbH
Pages: - Volume / Issue: 18 (19) Sequence Number: - Start / End Page: 2919 - 2929 Identifier: ISSN: 1616-301X
CoNE: https://pure.mpg.de/cone/journals/resource/954925596563