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  Actin-cytoskeleton dynamics in non-monotonic cell spreading

Heinrich, D., Youssef, S., Schroth-Diez, B., Engel, U., Aydin, D., Blümmel, J., et al. (2008). Actin-cytoskeleton dynamics in non-monotonic cell spreading. Cell adhesion & migration, 2(2), 58-68. doi:10.4161/cam.2.2.6190.

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 Creators:
Heinrich, Doris, Author
Youssef, Simon, Author
Schroth-Diez, Britta, Author
Engel, Ulrike, Author
Aydin, Daniel1, Author              
Blümmel, Jacques1, Author              
Spatz, Joachim P.1, 2, Author              
Gerisch, Günther, Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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Free keywords: actin cytoskeleton, Arp 2/3 complex, cell adhesion, cell spreading, Coronin, Dictyostelium, myosin, self-organization, clathrin
 Abstract: The spreading of motile cells on a substrate surface is accompanied by reorganization of their actin network. We show that spreading in the highly motile cells of Dictyostelium is non-monotonic, and thus differs from the passage of spreading cells through a regular series of stages. Quantification of the gain and loss of contact area revealed fluctuating forces of protrusion and retraction that dominate the interaction of Dictyostelium cells with a substrate. The molecular basis of these fluctuations is elucidated by dual-fluorescence labeling of filamentous actin together with proteins that highlight specific activities in the actin system. Front-to-tail polarity is established by the sorting out of myosin-II from regions where dense actin assemblies are accumulating. Myosin-IB identifies protruding front regions, and the Arp2/3 complex localizes to lamellipodia protruded from the fronts. Coronin is used as a sensitive indicator of actin disassembly to visualize the delicate balance of polymerization and depolymerization in spreading cells. Short-lived actin patches that co-localize with clathrin suggest that membrane internalization occurs even when the substrate-attached cell surface expands. We conclude that non-monotonic cell spreading is characterized by spatiotemporal patterns formed by motor proteins together with regulatory proteins that either promote or terminate actin polymerization on the scale of seconds.

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Language(s): eng - English
 Dates: 2008-05-032008-04-232008-04-232008
 Publication Status: Published in print
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Cell adhesion & migration
Source Genre: Journal
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Publ. Info: Austin, Tex. : Landes Bioscience
Pages: - Volume / Issue: 2 (2) Sequence Number: - Start / End Page: 58 - 68 Identifier: CoNE: https://pure.mpg.de/cone/journals/resource/http://purl.org/escidoc/metadata/terms/0.1/ISSN
ISSN: 1933-6918