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  Mapping the mechanics and macromolecular organization of hyaluronan-rich cell coats

Böhm, H., Mundinger, T. A., Boehm, C., Hagel, V., Rauch, U., Spatz, J. P., et al. (2009). Mapping the mechanics and macromolecular organization of hyaluronan-rich cell coats. Soft Matter, 5, 4331-4337. doi:10.1039/b905574f.

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 Creators:
Böhm, Heike1, 2, Author           
Mundinger, Tabea A.1, 2, Author           
Boehm, Christian1, 2, Author           
Hagel, Valentin, Author
Rauch, Uwe, Author
Spatz, Joachim P.1, 2, Author           
Curtis, Jennifer E.1, Author           
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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 Abstract: The hyaluronan (HA)-rich pericellular coat (PCC) enveloping most mammalian cells plays a vital role in biological processes such as cell adhesion, proliferation, motility and embryogenesis. In particular its presence on chondrocytes, which live in the load-bearing cartilage, has a wide range of implications in diseases such as osteoarthritis, highlighting its mechanical role in living organisms. Despite its significance, the macromolecular organization of the cell coat remains speculative. In order to obtain a more detailed spatial picture of highly hydrated PCCs, we present two independent but complementary non-invasive techniques for the position-resolved analysis of the cell coat's mechanical and structural properties. Position-dependent microrheology provides a micromechanical map of the PCC that reveals a gradient of increasing elastic stiffness towards the plasma membrane on model rat chondrocyte cells (RCJ-P). This gradient can be correlated with the relative distribution of HA, which is inferred using an eGFP-labelled neurocan-binding domain, a small fluorescent molecule that binds to HA. The spatial variation of the HA concentration profile is consistent with the position-dependent elasticity. Combining these approaches sheds light on the molecular architecture of the PCC.

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Language(s): eng - English
 Dates: 2009-03-202009-07-142009-09-072009
 Publication Status: Published in print
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 437389
DOI: 10.1039/b905574f
 Degree: -

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Title: Soft Matter
  Abbreviation : Soft Matter
Source Genre: Journal
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Publ. Info: Cambridge, UK : Royal Society of Chemistry
Pages: - Volume / Issue: 5 Sequence Number: - Start / End Page: 4331 - 4337 Identifier: ISSN: 1744-683X
CoNE: https://pure.mpg.de/cone/journals/resource/1744-683X