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  Polymeric substrates with tunable elasticity and nanoscopically controlled biomolecule presentation

Aydin, D., Louban, I., Perschmann, N., Blümmel, J., Lohmueller, T., Cavalcanti-Adam, E. A., et al. (2010). Polymeric substrates with tunable elasticity and nanoscopically controlled biomolecule presentation. Langmuir, 26(19), 15472-15480. doi:10.1021/la103065x.

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 Creators:
Aydin, Daniel1, Author              
Louban, Ilia1, Author              
Perschmann, Nadine1, 2, Author              
Blümmel, Jacques1, Author              
Lohmueller, Theobald1, Author              
Cavalcanti-Adam, Elisabetta Ada1, 2, Author              
Haas, Tobias L., Author
Walczak, Henning, Author
Kessler, Horst, Author
Fiammengo, Roberto1, Author              
Spatz, Joachim P.1, 2, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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 Abstract: Despite tremendous progress in recent years, nanopatterning of hydrated polymeric systems such as hydrogels still represents a major challenge. Here, we employ block copolymer nanolithography to arrange gold nanoparticles on a solid template, followed by the transfer of the pattern to a polymeric hydrogel. In the next step, these nanoparticles serve as specific anchor points for active biomolecules. We demonstrate the engineering of poly(ethylene glycol) hydrogel surfaces with respect to elasticity, nanopatterning, and functionalization with biomolecules. For the first time, biomolecule arrangement on the nanometer scale and substrate stiffness can be varied independently from each other. Young’s moduli, a measure of the compliance of the substrates, can be tuned over 4 orders of magnitude, including the values for all of the different tissues found in the human body. Structured hydrogels can be used to pattern any histidine-tagged protein as exemplified for his-protein A as an acceptor for immunoglobulin. When cell-adhesion-promoting peptide cRGDfK is selectively coupled to gold nanoparticles, the surfaces provide cues for cell−surface interaction and allow for the study of the modulation of cellular adhesion by the mechanical properties of the environment. Therefore, these substrates represent a unique multipurpose platform for studying receptor/ligand interactions with adhering cells, mechanotransduction, and cell-adhesion-dependent signaling.

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Language(s): eng - English
 Dates: 2010-08-272010-08-022010-09-132010
 Publication Status: Published in print
 Pages: 9
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 510632
DOI: 10.1021/la103065x
 Degree: -

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Title: Langmuir
Source Genre: Journal
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Publ. Info: Columbus, OH : American Chemical Society
Pages: - Volume / Issue: 26 (19) Sequence Number: - Start / End Page: 15472 - 15480 Identifier: ISSN: 0743-7463
CoNE: https://pure.mpg.de/cone/journals/resource/954925541194