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  Regulation of the P450 oxygenation cascade involved in glycopeptide antibiotic biosynthesis

Peschke, M., Haslinger, K., Brieke, C., Reinstein, J., & Cryle, M. J. (2016). Regulation of the P450 oxygenation cascade involved in glycopeptide antibiotic biosynthesis. Journal of the American Chemical Society, 138(21), 6746-6753. doi:10.1021/jacs.6b00307.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-002C-1F68-1 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-002C-1F7D-6
Genre: Journal Article

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 Creators:
Peschke, Madeleine1, Author           
Haslinger, Kristina1, Author           
Brieke, Clara1, Author           
Reinstein, Jochen1, Author           
Cryle, Max J.1, Author           
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1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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 Abstract: Glycopeptide antibiotics (GPAs) are nonribosomal peptides rich in modifications introduced by external enzymes. These enzymes act on the free peptide aglycone or intermediates bound to the nonribosomal peptide synthetase (NRPS) assembly line. In this process the terminal module of the NRPS plays a crucial role as it contains a unique recruitment platform (X-domain) interacting with three to four modifying Cytochrome P450 (P450) enzymes that are responsible for cyclizing bound peptides. However, whether these enzymes share the same binding site on the X-domain and how the order of the cyclization steps is orchestrated has remained elusive. In this study we investigate the first two reactions in teicoplanin aglycone maturation catalyzed by the enzymes OxyBtei and OxyAtei. We demonstrate that both enzymes interact with the X-domain via the identical interaction site with similar affinities, irrespective of the peptide modification stage, while their catalytic activity is restricted to the correctly cross-linked peptide. On the basis of steady state kinetics of the OxyBtei-catalyzed reaction, we propose a model for P450 recruitment and peptide modification that involves continuous association/dissociation of the P450 enzymes with the NRPS, followed by specific recognition of the peptide cyclization state by the P450 (scanning). This leads to an induced conformational change that enhances the affinity of the enzyme/substrate complex and initiates catalysis; product release then occurs, with the product itself becoming the substrate for the second enzyme in the pathway. This model rationalizes our experimental findings for this complex enzyme cascade and provides insights into the orchestration of the sequential peptide tailoring reactions on the terminal NRPS module in GPA biosynthesis.

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Language(s): eng - English
 Dates: 2016-01-102016-05-232016-06-01
 Publication Status: Issued
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1021/jacs.6b00307
URI: https://www.ncbi.nlm.nih.gov/pubmed/27213615
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Title: Journal of the American Chemical Society
  Other : J. Am. Chem. Soc.
  Abbreviation : JACS
Source Genre: Journal
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Publ. Info: Washington, DC : American Chemical Society
Pages: - Volume / Issue: 138 (21) Sequence Number: - Start / End Page: 6746 - 6753 Identifier: ISSN: 0002-7863
CoNE: https://pure.mpg.de/cone/journals/resource/954925376870