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  Characterizing Active Site Conformational Heterogeneity along the Trajectory of an Enzymatic Phosphoryl Transfer Reaction

Zeymer, C., Werbeck, N. D., Zimmermann, S., Reinstein, J., & Hansen, D. F. (2016). Characterizing Active Site Conformational Heterogeneity along the Trajectory of an Enzymatic Phosphoryl Transfer Reaction. Angewandte Chemie International Edition, 55(38), 11533-11537. doi:10.1002/anie.201606238.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-1F73-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-1F74-7
Genre: Journal Article

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AngewChemIntEd_55_2016_11533_Suppl.pdf (Supplementary material), 2MB
 
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 Creators:
Zeymer, Cathleen1, Author              
Werbeck, Nicolas D., Author
Zimmermann, Sabine1, Author              
Reinstein, Jochen1, Author              
Hansen, D. Flemming, Author
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1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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 Abstract: States along the phosphoryl transfer reaction catalyzed by the nucleoside monophosphate kinase UmpK were captured and changes in the conformational heterogeneity of conserved active site arginine side-chains were quantified by NMR spin-relaxation methods. In addition to apo and ligand-bound UmpK, a transition state analog (TSA) complex was utilized to evaluate the extent to which active site conformational entropy contributes to the transition state free energy. The catalytically essential arginine side-chain guanidino groups were found to be remarkably rigid in the TSA complex, indicating that the enzyme has evolved to restrict the conformational freedom along its reaction path over the energy landscape, which in turn allows the phosphoryl transfer to occur selectively by avoiding side reactions.

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Language(s): eng - English
 Dates: 2016-06-272016-08-182016-08-182016-09-12
 Publication Status: Published in print
 Pages: 5
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 Rev. Method: Peer
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Title: Angewandte Chemie International Edition
  Other : Angew. Chem., Int. Ed.
  Other : Angew. Chem. Int. Ed.
  Other : Angewandte Chemie, International Edition
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: 55 (38) Sequence Number: - Start / End Page: 11533 - 11537 Identifier: ISSN: 1433-7851
CoNE: https://pure.mpg.de/cone/journals/resource/1433-7851