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  The novel fluorescent CDP-analogue (Pβ)MABA-CDP is a specific probe for the NMP binding site of UMP/CMP-kinase

Rudolph, M. G., Veit, T., & Reinstein, J. (1999). The novel fluorescent CDP-analogue (Pβ)MABA-CDP is a specific probe for the NMP binding site of UMP/CMP-kinase. Protein Science, 8(12), 2697-2704. doi:10.1110/ps.8.12.2697.

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Genre: Journal Article
Alternative Title : The novel fluorescent CDP-analogue (Pbeta)MABA-CDP is a specific probe for the NMP binding site of UMP/CMP-kinase

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ProtSci_8_1999_2697.pdf (Any fulltext), 233KB
 
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 Creators:
Rudolph, Markus G., Author
Veit, Thomas, Author
Reinstein, Jochen1, Author           
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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Free keywords: fluorescence; kinases; kinetics; nucleotide analogues
 Abstract: Direct thermodynamic and kinetic investigations of the binding of nucleotides to the nucleoside monophosphate (NMP) site of NMP kinases have not been possible so far because a spectroscopic probe was not available. By coupling a fluorescent N-methylanthraniloyl- (mant) group to the β-phosphate of CDP via a butyl linker, a CDP analogue [(Pβ)MABA-CDP] was obtained that still binds specifically to the NMP site of UmpKdicty, because the base and the ribose moieties, which are involved in specific interactions, are not modified. This allows the direct determination of binding constants for its substrates in competition experiments.

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Language(s): eng - English
 Dates: 1999-07-131999-09-161999-12-01
 Publication Status: Issued
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 665718
DOI: 10.1110/ps.8.12.2697
Other: 6228
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Title: Protein Science
Source Genre: Journal
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Publ. Info: New York, N.Y. : Cambridge University Press
Pages: - Volume / Issue: 8 (12) Sequence Number: - Start / End Page: 2697 - 2704 Identifier: ISSN: 0961-8368
CoNE: https://pure.mpg.de/cone/journals/resource/954925342760