English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Total synthesis of dansylated Park's nucleotide for high-throughput MraY assays.

Wohnig, S., Anatol, P. S. A., Koppermann, S., Mieskes, G., Gisch, N., Jahn, R., et al. (2016). Total synthesis of dansylated Park's nucleotide for high-throughput MraY assays. Chemistry - A European Journal, 22(49), 17813-17819. doi:10.1002/chem.201604279.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-241D-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-296B-F
Genre: Journal Article

Files

show Files
hide Files
:
2376029.pdf (Publisher version), 2MB
 
File Permalink:
-
Name:
2376029.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Biophysical Chemistry (Karl Friedrich Bonhoeffer Institute), Göttingen; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2376029_Suppl.pdf (Supplementary material), 3MB
Name:
2376029_Suppl.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Wohnig, S., Author
Anatol, P. S. A., Author
Koppermann, S., Author
Mieskes, G.1, Author              
Gisch, N., Author
Jahn, R.1, Author              
Ducho, C., Author
Affiliations:
1Department of Neurobiology, MPI for biophysical chemistry, Max Planck Society, ou_578595              

Content

show
hide
Free keywords: Antibiotics; Enzyme assays; Fluorescence; Medicinal chemistry; Peptidoglycan
 Abstract: The membrane protein translocase I (MraY) is a key enzyme in bacterial peptidoglycan biosynthesis. It is therefore frequently discussed as a target for the development of novel antibiotics. The screening of compound libraries for the identification of MraY inhibitors is enabled by an established fluorescence-based MraY assay. However, this assay requires a dansylated derivative of the bacterial biosynthetic intermediate Park's nucleotide as the MraY substrate. Isolation of Park's nucleotide from bacteria and subsequent dansylation only furnishes limited amounts of this substrate, thus hampering the high-throughput screening for MraY inhibitors. Accordingly, the efficient provision of dansylated Park's nucleotide is a major bottleneck in the exploration of this promising drug target. In this work, we present the first total synthesis of dansylated Park's nucleotide, affording an unprecedented amount of the target compound for high-throughput MraY assays.

Details

show
hide
Language(s): eng - English
 Dates: 2016-10-282016-12-05
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1002/chem.201604279
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Chemistry - A European Journal
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 22 (49) Sequence Number: - Start / End Page: 17813 - 17819 Identifier: -