English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Patch clamp techniques used for studying synaptic transmission in slices of mammalian brain.

Sakmann, B., Edwards, F., Konnerth, A., & Takahashi, T. (1989). Patch clamp techniques used for studying synaptic transmission in slices of mammalian brain. Quarterly Journal of Experimental Physiology, 74(6), 1107-1118. doi:10.1113/expphysiol.1989.sp003336.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-270A-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-270E-1
Genre: Journal Article

Files

show Files
hide Files
:
2376414.pdf (Publisher version), 3MB
Name:
2376414.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Sakmann, B.1, Author              
Edwards, F., Author
Konnerth, A.2, Author              
Takahashi, T., Author
Affiliations:
1Abteilung Zellphysiologie, MPI for biophysical chemistry, Max Planck Society, ou_578558              
2Research Group of Cellular Neurophysiology, MPI for biophysical chemistry, Max Planck Society, ou_578557              

Content

show
hide
Free keywords: -
 Abstract: Procedures are described for recording postsynaptic currents from neurones in slices of rat brain using patch clamp techniques. The method involves cutting brain slices (120-300, µm thick) with a vibrating microtome followed by localization of cell somata, which can be clearly seen with Nomarski differential interference contrast optics in the light microscope. Tissue covering the identified cell is then removed mechanically and standard patch clamp techniques are applied. Using these methods, spontaneously occurring and stimulus-evoked inhibitory postsynaptic currents (IPSCs) were recorded from neurones in rat hippocampus at greatly improved resolution. In the presence of tetrodotoxin, to block presynaptic action potentials, spontaneous IPSCs seldom exceeded 25 pA. Evoked IPSCs elicited by constant electrical stimulation of a presynaptic neurone were larger and fluctuated in their amplitudes. Single-channel currents, activated by the putative inhibitory transmitter γ-aminobutyric acid (GABA), had a size of about 1 pA. The number of postsynaptic channels activated by a packet of inhibitory transmitter is probably not more than thirty, nearly two orders of magnitude smaller than previously reported estimates for CNS synapses. This might reflect matching of synaptic efficacy to the high input resistance of hippocampal neurones and could be a requirement for fine tuning of inhibition.

Details

show
hide
Language(s): eng - English
 Dates: 1989-11-121989
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1113/expphysiol.1989.sp003336
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Quarterly Journal of Experimental Physiology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 74 (6) Sequence Number: - Start / End Page: 1107 - 1118 Identifier: -