English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Fluorescence energy transfer shows that the four-way DNA junction is a right-handed cross of antiparallel molecules.

Murchie, A. I. H., Clegg, R. M., von Krtzing, E., Duckett, D. R., Diekmann, S., & Lilley, D. M. (1989). Fluorescence energy transfer shows that the four-way DNA junction is a right-handed cross of antiparallel molecules. Nature, 341(6244), 763-766. doi:10.1038/341763a0.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-2A07-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-2AA7-F
Genre: Journal Article

Files

show Files
hide Files
:
2376737.pdf (Publisher version), 466KB
Name:
2376737.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Murchie, A. I. H., Author
Clegg, R. M.1, Author              
von Krtzing, E., Author
Duckett, D. R., Author
Diekmann, S.1, Author              
Lilley, D. M., Author
Affiliations:
1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              

Content

show
hide
Free keywords: -
 Abstract: THE four-way junction between DNA helices is the central intermediate in recombination1–10, and the manner of its interaction with resolvase enzymes can determine the genetic outcome of the process. A knowledge of its structure is a prerequisite to understanding the interaction with proteins, and there has been recent progress11–14. Here we use fluorescence energy transfer to determine the relative distances between the ends of a small DNA junction, and hence the path of the strands. Our results are consistent with the geometry of an 'X'. The interconnected helices are juxtaposed so that the continuous strands of each helix generate an antiparallel alignment, and the two interchanged strands do not cross at the centre. The acute angle of the X structure is defined by a right-handed rotation of the helical axes about the axis perpendicular to the X plane, as viewed from the centre of the X.

Details

show
hide
Language(s): eng - English
 Dates: 1989-10-26
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1038/341763a0
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 341 (6244) Sequence Number: - Start / End Page: 763 - 766 Identifier: -