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  Developmentally regulated epitopes on a neurofilament protein visualized by monoclonal antibodies.

Thorey, I., & Seifert, W. (1989). Developmentally regulated epitopes on a neurofilament protein visualized by monoclonal antibodies. Developmental Brain Research, 49(2), 229-241. doi:10.1016/0165-3806(89)90024-2.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-2AEC-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-2AF0-B
Genre: Journal Article

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2376873.pdf (Publisher version), 3MB
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 Creators:
Thorey, I.1, Author              
Seifert, W.1, Author              
Affiliations:
1Abteilung Neurobiologie, MPI for biophysical chemistry, Max Planck Society, ou_578620              

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Free keywords: Monoclonal antibody; Developmentally regulated antigen; Cytoskeleton; Neurofilament; Microheterogeneity
 Abstract: Two monoclonal antibodies (mabs) which recognized the 68 kDa subunit of the rat neurofilament triplet were isolated. In immunoblots with SDS-solubilized and reduced proteins, these mabs recognized their epitopes equally well in embryonic and in adult tissue. However, these epitopes were developmentally regulated in paraformaldehyde-fixed rat brain sections. They were abundant in all compartments of differentiating neurons, whereas in mature neurons their presence was markedly attenuated, with a moderate abundance in perikarya and larger dendrites and low concentrations in axons. Thus, a differential developmental modification, possibly involving the masking of an epitope, is demonstrated for the small neurofilament polypeptide in rat and monkey brain tissue.

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Language(s): eng - English
 Dates: 2003-04-141989-10-01
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/0165-3806(89)90024-2
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Title: Developmental Brain Research
Source Genre: Journal
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Pages: - Volume / Issue: 49 (2) Sequence Number: - Start / End Page: 229 - 241 Identifier: -