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  Isolation and DNA-binding characteristics of a protein involved in transcription activation of two divergently transcribed, essential yeast genes.

Halfter, H., Müller, U., Winnacker, E. L., & Gallwitz, D. (1989). Isolation and DNA-binding characteristics of a protein involved in transcription activation of two divergently transcribed, essential yeast genes. EMBO Journal, 8(10), 3029-3037.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-2AF1-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-2AF4-3
Genre: Journal Article

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 Creators:
Halfter, H.1, Author              
Müller, U., Author
Winnacker, E. L., Author
Gallwitz, D.1, Author              
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1Department of Molecular Genetics, MPI for biophysical chemistry, Max Planck Society, ou_578622              

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 Abstract: We have identified a protein, BAF1, which has two oppositely oriented, partially overlapping binding sites within a symmetrical sequence located midway between and upstream of the divergently transcribed YPT1 and TUB2 genes of the yeast Saccharomyces cerevisiae. The 120 kd BAF1 protein was purified to near homogeneity and used to delineate the two binding sites and to identify apparent protein contact sites by the missing contact technique, methylation interference and by site-directed mutagenesis. The BAF1-recognition sequence contains a conserved TCN7ACG element recently identified at autonomously replicating sequences (ARS) and in the 5' and 3' flanking region of other yeast genes. The symmetrical sequence of the YPT1/TUB2 intergene region seems not to be involved in DNA replication but activates transcription in an orientation-independent fashion.

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Language(s): eng - English
 Dates: 1989-10
 Publication Status: Published in print
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Title: EMBO Journal
Source Genre: Journal
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Pages: - Volume / Issue: 8 (10) Sequence Number: - Start / End Page: 3029 - 3037 Identifier: -