非表示:
キーワード:
Stress, fear, VTA, CRF, memory
要旨:
The neuropeptide corticotropin-releasing factor (CRF) coordinates the
physiological and behavioural responses to stress. CRF receptors are
highly expressed in the ventral tegmental area (VTA), an important
region for motivated behaviour. Therefore, we examined the role of CRF
receptor type 1 (CRFR1) in the VTA in conditioned fear, using a
viral-mediated RNA interference approach. Following stereotaxic
injection of a lentivirus that contained either shCRF-R1 or a control
sequence, mice received tone-footshock pairings. Intra-VTA shCRF-R1 did
not affect tone-elicited freezing during conditioning. Once conditioned
fear was acquired, however, shCRF-R1 mice consistently showed stronger
freezing to the tone even after extinction and reinstatement. These
results implicate a novel role of VTA CRF-R1 in conditioned fear, and
suggest how stress may modulate aversive learning and memory. (C) 2016
Elsevier B.V. and ECNP. All rights reserved.
