ausblenden:
Schlagwörter:
RNA-seq; Single nucleotide polymorphism; Norway spruce; Genotyping
Zusammenfassung:
A novel set of SNPs was derived from transcriptome
data of ten Norway spruce (Picea abies) trees
from the Bavarian Forest National Park in Germany
(BaFoNP). SNPs were identified by mapping against
a de-novo transcriptome assembly and against pre-mRNAs
of predicted genes of the reference genome assembly. This
resulted in 111,849 and 366,577 SNPs, respectively. Out of
these, 311 were either randomly selected or chosen because
of their pronounced divergence between sampling sites and
genotyped in 218 trees with an Illumina Infinium HD
iSelect BeadChip.
