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  Surface Curvature Differentially Regulates Stem Cell Migration and Differentiation via Altered Attachment Morphology and Nuclear Deformation

Werner, M., Blanquer, S. B. G., Haimi, S. P., Korus, G., Dunlop, J. W. C., Duda, G. N., et al. (2017). Surface Curvature Differentially Regulates Stem Cell Migration and Differentiation via Altered Attachment Morphology and Nuclear Deformation. Advanced Science, 4(2): 1600347. doi:10.1002/advs.201600347.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-3294-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-978B-F
Genre: Journal Article

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 Creators:
Werner, Maike, Author
Blanquer, Sébastien B. G., Author
Haimi, Suvi P., Author
Korus, Gabriela, Author
Dunlop, John W. C.1, Author              
Duda, Georg N., Author
Grijpma, Dirk. W., Author
Petersen, Ansgar, Author
Affiliations:
1John Dunlop, Biomaterialien, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863291              

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Free keywords: 3D surface curvature, cell migration, nuclear mechanics, osteogenic differentiation, stereolithography, Open Access
 Abstract: Signals from the microenvironment around a cell are known to influence cell behavior. Material properties, such as biochemical composition and substrate stiffness, are today accepted as significant regulators of stem cell fate. The knowledge of how cell behavior is influenced by 3D geometric cues is, however, strongly limited despite its potential relevance for the understanding of tissue regenerative processes and the design of biomaterials. Here, the role of surface curvature on the migratory and differentiation behavior of human mesenchymal stem cells (hMSCs) has been investigated on 3D surfaces with well-defined geometric features produced by stereolithography. Time lapse microscopy reveals a significant increase of cell migration speed on concave spherical compared to convex spherical structures and flat surfaces resulting from an upward-lift of the cell body due to cytoskeletal forces. On convex surfaces, cytoskeletal forces lead to substantial nuclear deformation, increase lamin-A levels and promote osteogenic differentiation. The findings of this study demonstrate a so far missing link between 3D surface curvature and hMSC behavior. This will not only help to better understand the role of extracellular matrix architecture in health and disease but also give new insights in how 3D geometries can be used as a cell-instructive material parameter in the field of biomaterial-guided tissue regeneration.

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 Dates: 2016-112017
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1002/advs.201600347
 Degree: -

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Title: Advanced Science
  Other : Adv. Sci.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: 4 (2) Sequence Number: 1600347 Start / End Page: - Identifier: ISSN: 2198-3844