English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  γ-Aminobutyric acid elevates cytosolic Ca in bovine chromaffin cells.

Doroshenko, P. (1989). γ-Aminobutyric acid elevates cytosolic Ca in bovine chromaffin cells. Neuroscience Letters, 104(1-2), 83-87. doi:10.1016/0304-3940(89)90333-9.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-2E68-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-2E6B-6
Genre: Journal Article

Files

show Files
hide Files
:
2378146.pdf (Publisher version), 294KB
Name:
2378146.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Doroshenko, P.A.1, Author              
Affiliations:
1Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society, ou_578579              

Content

show
hide
Free keywords: Chromaffin cell; γ-Aminobutyric acid; Cytosolic Ca; Fura-2; Calcium channel; Bicuculline; Bovine adrenal medulla
 Abstract: Measurements of the cytosolic Ca concentration ([Ca]i) with the Ca-sensitive dye, fura-2, showed that in intact, but not in voltage-clamped, bovine chromaffin cells γ-aminobutyric acid (GABA, 10 μM) elicited a transient increase in [Ca]i. The Ca transient of intact cells was inhibited by bicuculline (20 μM), by removal of extracellular Ca or by treatment with the Ca channel blocker cobalt (2.5 mM), and enhanced by lowering the extracellular Cl. We conclude, that GABA elevates [Ca]i by inducing a GABAA-receptor-linked Cl current which depolarizes the cell membrane sufficiently to activate potential-operated Ca channels and cause Ca entry into the cell.

Details

show
hide
Language(s): eng - English
 Dates: 2003-03-181989-09-25
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/0304-3940(89)90333-9
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Neuroscience Letters
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 104 (1-2) Sequence Number: - Start / End Page: 83 - 87 Identifier: -