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  Spatial and temporal dynamics of pacific oyster hemolymph microbiota across multiple scales

Lokmer, A., Goedknegt, M., Thieltges, D. W., Fiorentino, D., Künzel, S., Baines, J. F., et al. (2016). Spatial and temporal dynamics of pacific oyster hemolymph microbiota across multiple scales. Frontiers in Microbiology, 7(AUG): Article 1367. doi:10.3389/fmicb.2016.01367.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-3AE6-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-1F67-6
Genre: Journal Article

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 Creators:
Lokmer, Ana, Author
Goedknegt, M.Anouk, Author
Thieltges, David W., Author
Fiorentino, Dario, Author
Künzel, Sven1, Author              
Baines, John F.2, Author              
Wegner, K. Mathias, Author
Affiliations:
1Department Evolutionary Genetics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445635              
2Guest Group Evolutionary Genomics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445638              

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Free keywords: amplicon; antibiotic therapy; Crassostrea gigas; genetic background; hemolymph; human; human tissue; microbial community; microbiome; nonhuman; resident; stratified sample; summer
 Abstract: Unveiling the factors and processes that shape the dynamics of host associated microbial communities (microbiota) under natural conditions is an important part of understanding and predicting an organism's response to a changing environment. The microbiota is shaped by host (i.e., genetic) factors as well as by the biotic and abiotic environment. Studying natural variation of microbial community composition in multiple host genetic backgrounds across spatial as well as temporal scales represents a means to untangle this complex interplay. Here, we combined a spatially-stratified with a longitudinal sampling scheme within differentiated host genetic backgrounds by reciprocally transplanting Pacific oysters between two sites in the Wadden Sea (Sylt and Texel). To further differentiate contingent site from host genetic effects, we repeatedly sampled the same individuals over a summer season to examine structure, diversity and dynamics of individual hemolymph microbiota following experimental removal of resident microbiota by antibiotic treatment. While a large proportion of microbiome variation could be attributed to immediate environmental conditions, we observed persistent effects of antibiotic treatment and translocation suggesting that hemolymph microbial community dynamics is subject to within-microbiome interactions and host population specific factors. In addition, the analysis of spatial variation revealed that the within-site microenvironmental heterogeneity resulted in high small-scale variability, as opposed to large-scale (between-site) stability. Similarly, considerable within-individual temporal variability was in contrast with the overall temporal stability at the site level. Overall, our longitudinal, spatially-stratified sampling design revealed that variation in hemolymph microbiota is strongly influenced by site and immediate environmental conditions, whereas internal microbiome dynamics and oyster-related factors add to their long-term stability. The combination of small and large scale resolution of spatial and temporal observations therefore represents a crucial but underused tool to study host-associated microbiome dynamics. © 2016 Lokmer, Goedknegt, Thieltges, Fiorentino, Kuenzel, Baines and Wegner.

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Language(s): eng - English
 Dates: 2016-04-272016-08-182016-08-312016
 Publication Status: Published in print
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 Rev. Method: -
 Identifiers: DOI: 10.3389/fmicb.2016.01367
BibTex Citekey: Lokmer2016
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Title: Frontiers in Microbiology
  Abbreviation : Front. Microbiol.
Source Genre: Journal
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Publ. Info: Lausanne : Frontiers Media
Pages: - Volume / Issue: 7 (AUG) Sequence Number: Article 1367 Start / End Page: - Identifier: ISSN: 1664-302X
CoNE: https://pure.mpg.de/cone/journals/resource/1664-302X