English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
 Local TagsRelease HistoryDetailsSummary
  Lineage tracking for probing heritable phenotypes at single-cell resolution

Cottinet, D., Condamine, F., Bremond, N., Griffiths, A., Rainey, P., De Visser, J., et al. (2016). Lineage tracking for probing heritable phenotypes at single-cell resolution. PLoS ONE, 11(4). doi:10.1371/journal.pone.0152395.

Item is

 

show all hide all

Basic

show hide
Item Permalink: https://hdl.handle.net/11858/00-001M-0000-002C-3AEF-1 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-002C-3AF1-A
Genre: Journal Article

Files

show Files

Locators

show
hide
Locator:
Link (Any fulltext)
Description:
-
OA-Status:

Creators

show
hide
 Creators:
Cottinet, D.1, Author
Condamine, F., Author
Bremond, N., Author
Griffiths, A.D., Author
Rainey, P.B., Author
De Visser, J.A.G.M., Author
Baudry, J., Author
Bibette, J., Author
Affiliations:
1External Organizations, ou_persistent22              

Content

show
hide
Free keywords: DNA sequence; Escherichia coli; microbiology; mutation; phenotype; population biology; starvation
 Abstract: Determining the phenotype and genotype of single cells is central to understand microbial evolution. DNA sequencing technologies allow the detection of mutants at high resolution, but similar approaches for phenotypic analyses are still lacking. We show that a drop-based millifluidic system enables the detection of heritable phenotypic changes in evolving bacterial populations. At time intervals, cells were sampled and individually compartmentalized in 100 nL drops. Growth through 15 generations was monitored using a fluorescent protein reporter. Amplification of heritable changes-via growth-over multiple generations yields phenotypically distinct clusters reflecting variation relevant for evolution. To demonstrate the utility of this approach, we follow the evolution of Escherichia coli populations during 30 days of starvation. Phenotypic diversity was observed to rapidly increase upon starvation with the emergence of heritable phenotypes. Mutations corresponding to each phenotypic class were identified by DNA sequencing. This scalable lineage-tracking technology opens the door to large-scale phenotyping methods with special utility for microbiology and microbial population biology. © 2016 Cottinet et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Details

show
hide
Language(s):
 Dates: 2016
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1371/journal.pone.0152395
BibTex Citekey: Cottinet2016
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: PLoS ONE
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Public Library of Science
Pages: - Volume / Issue: 11 (4) Sequence Number: - Start / End Page: - Identifier: -