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  Mixed-linkage glucan oligosaccharides produced by automated glycan assembly serve as tools to determine the substrate specificity of lichenase

Dallabernardina, P., Schuhmacher, F., Seeberger, P. H., & Pfrengle, F. (2017). Mixed-linkage glucan oligosaccharides produced by automated glycan assembly serve as tools to determine the substrate specificity of lichenase. Chemistry – A European Journal, 23(13), 3191-3196. doi:10.1002/chem.201605479.

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 Urheber:
Dallabernardina, Pietro1, Autor           
Schuhmacher, Frank2, Autor           
Seeberger, Peter H.2, Autor           
Pfrengle, Fabian1, Autor           
Affiliations:
1Fabian Pfrengle, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863303              
2Peter H. Seeberger - Automated Systems, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863306              

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Schlagwörter: automated glycan assembly, plant cell wall, mixed-linkage glucan
 Zusammenfassung: The mixed-linkage (1→3),(1→4)-D-glucan (MLG) specific glycosyl hydrolase lichenase is an important biochemical tool for the structural characterization of MLGs. It holds potential for application in the brewery, animal feed, and biofuel industries. Several defined MLG oligosaccharides obtained by automated glycan assembly are used to analyze the substrate specificities of Bacillus subtilis lichenase. Two glucose building blocks (BBs), equipped with a temporary Fmoc protecting group in the C-3 or C-4 position, served to assemble different oligosaccharides using an automated oligosaccharide synthesizer. Light-induced cleavage of the glycan products from the solid support followed by global deprotection provided seven MLG oligosaccharides of different length and connectivity. After incubation of the MLG oligosaccharides with lichenase, the digestion products were analyzed by HPLC-MS. These digestion experiments provided insights into the enzyme's active site that is in line with other recent evidence suggesting that the substrate specificity of lichenases has to be reconsidered. These results demonstrate that synthetic MLG oligosaccharides are useful tools to analyse mixed-linkage β-glucanases.

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Sprache(n): eng - English
 Datum: 2017-01-132017
 Publikationsstatus: Erschienen
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 Identifikatoren: DOI: 10.1002/chem.201605479
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Titel: Chemistry – A European Journal
  Andere : Chem. – Eur. J.
  Andere : Chem. Eur. J.
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Weinheim : Wiley-VCH
Seiten: - Band / Heft: 23 (13) Artikelnummer: - Start- / Endseite: 3191 - 3196 Identifikator: ISSN: 0947-6539