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  Immunologisch aktive Glykoproteine aus Baptisia tinctoria.

Beuscher, N., Scheit, K., Bodinet, C., & Kopanski, L. (1989). Immunologisch aktive Glykoproteine aus Baptisia tinctoria. Planta Medica, 55(4), 358-363. doi:10.1055/s-2006-962028.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-3E0A-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-3E0D-8
Genre: Journal Article

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Beuscher, N., Author
Scheit, K.H.1, Author              
Bodinet, C., Author
Kopanski, L., Author
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1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              

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 Abstract: Chromatographically purified fractions of aqueous-ethanolic extracts from Baptisia tinctoria roots contained a strong lymphocyte DNA synthesis-stimulating activity. Electrophoretic analysis of these fractions revealed four distinct protein bands with molecular masses of P 1 = 58 kD; P4 = 31 kD; P 5 = 26 kD; and P 6 = 14 kD. They contained carbohydrate as determined by periodic acid Schiff staining. An estimation of the approximate amount of sugar was done by using human transferrin as a reference, this method revealed the following values: P 1 = 27%; P 4 = 12%; P 5 = 14%; and P 6 = 8%. The mixture of proteins and every single band were immunoreactive with a polyclonal antiserum against Baptisia proteins determined in immune and dot blots, respectively. Electrophoretically purified proteins were characterized by tryptic cleavage and determination of their amino acid content. They contained several common amino acids, predominantly aspartic acid, glutamic acid, threonine, and alanine. The content of glucosamine and/or galactosamine was less than 0.2 Molper cent. The four proteins revealed pi values between 5.3 and 4.7. Protein P 4 was immunochemically related to phytohemagglutinin but, in contrast to PHA-P, it exhibited no hemagglutinating activity and no leucagglutinating activity like PHA-L.

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Language(s): deu - German
 Dates: 1989
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1055/s-2006-962028
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Title: Planta Medica
Source Genre: Journal
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Pages: - Volume / Issue: 55 (4) Sequence Number: - Start / End Page: 358 - 363 Identifier: -