English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Modeling sRNA-Regulated Plasmid Maintenance

Gong, C. C., & Klumpp, S. (2017). Modeling sRNA-Regulated Plasmid Maintenance. PLoS One, 12(1): e0169703. doi:10.1371/journal.pone.0169703.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-3FBE-1 Version Permalink: http://hdl.handle.net/21.11116/0000-0004-E308-3
Genre: Journal Article

Files

show Files
hide Files
:
2382854.pdf (Publisher version), 4MB
Name:
2382854.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
:
Preprint.pdf (Any fulltext), 5MB
Name:
Preprint.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-

Locators

show

Creators

show
hide
 Creators:
Gong, Chen Chris1, Author              
Klumpp, Stefan1, Author              
Affiliations:
1Stefan Klumpp, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863329              

Content

show
hide
Free keywords: Open Access
 Abstract: -

Details

show
hide
Language(s):
 Dates: 2017-01-132017
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: We study a theoretical model for the toxin-antitoxin (hok/sok) mechanism for plasmid maintenance in bacteria. Toxin-antitoxin systems enforce the maintenance of a plasmid through post-segregational killing of cells that have lost the plasmid. Key to their function is the tight regulation of expression of a protein toxin by an sRNA antitoxin. Here, we focus on the nonlinear nature of the regulatory circuit dynamics of the toxin-antitoxin mechanism. The mechanism relies on a transient increase in protein concentration rather than on the steady state of the genetic circuit. Through a systematic analysis of the parameter dependence of this transient increase, we confirm some known design features of this system and identify new ones: for an efficient toxin-antitoxin mechanism, the synthesis rate of the toxin's mRNA template should be lower that of the sRNA antitoxin, the mRNA template should be more stable than the sRNA antitoxin, and the mRNA-sRNA complex should be more stable than the sRNA antitoxin. Moreover, a short half-life of the protein toxin is also beneficial to the function of the toxin-antitoxin system. In addition, we study a therapeutic scenario in which a competitor mRNA is introduced to sequester the sRNA antitoxin, causing the toxic protein to be expressed.
 Rev. Method: Peer
 Identifiers: DOI: 10.1371/journal.pone.0169703
arXiv: https://arxiv.org/abs/1609.00977
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: PLoS One
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: San Francisco, CA : Public Library of Science
Pages: - Volume / Issue: 12 (1) Sequence Number: e0169703 Start / End Page: - Identifier: ISSN: 1932-6203