English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Functional interplay between MSL1 and CDK7 controls RNA polymerase II Ser5 phosphorylation

Chlamydas, S., Holz, H., Samata, M., Chelmicki, T., Georgiev, P., Pelechano, V., et al. (2016). Functional interplay between MSL1 and CDK7 controls RNA polymerase II Ser5 phosphorylation. Nature Structural and Molecular Biology, 23, 580-589. doi:10.1038/nsmb.3233.

Item is

Files

show Files
hide Files
:
chlamydas et al..pdf (Publisher version), 3MB
 
File Permalink:
-
Name:
chlamydas et al..pdf
Description:
-
Visibility:
Restricted (Max Planck Institute of Immunobiology and Epigenetics, MFIB; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Locator:
https://www.nature.com/articles/nsmb.3233 (Publisher version)
Description:
-

Creators

show
hide
 Creators:
Chlamydas, Sarantis1, Author
Holz, Herbert1, Author
Samata, Maria 1, 2, Author
Chelmicki, Tomasz1, Author
Georgiev, Plamen1, Author              
Pelechano, Vicent3, 4, Author
Dündar, Friederike1, 2, Author
Dasmeh, Pouria1, Author
Mittler, Gerhard1, Author              
Tavares Cadete, Filipe5, Author
Ramirez, Fidel1, Author
Conrad, Thomas1, Author
Wei, Wu3, 6, Author
Raja, Sunil1, Author
Manke, Thomas1, Author              
Luscombe, Nicholas M.5, 7, Author
Steinmetz, Lars M.3, 6, Author
Akhtar, Asifa1, Author              
Affiliations:
1Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, 79108 Freiburg, DE, ou_2243640              
2University of Freiburg, Faculty of Biology, Freiburg, Germany, ou_persistent22              
3Genome Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany, ou_persistent22              
4SciLifeLab, Department of Microbiology, Tumor and cell Bilogy, Karolinksa Institutet, Solna, Sweden, ou_persistent22              
5The Francis Crick Institute, London, UK, ou_persistent22              
6Stanford Genome Technology Center, Stanford University, California, USA, ou_persistent22              
7UCL Genetics Institute, Department of Genetics, Evolution and Environment, University College London, London, UK, ou_persistent22              

Content

show
hide
Free keywords: -
 Abstract: Proper gene expression requires coordinated interplay among transcriptional coactivators, transcription factors and the general transcription machinery. We report here that MSL1, a central component of the dosage compensation complex in Drosophila melanogaster and Drosophila virilis, displays evolutionarily conserved sex-independent binding to promoters. Genetic and biochemical analyses reveal a functional interaction of MSL1 with CDK7, a subunit of the Cdk-activating kinase (CAK) complex of the general transcription factor TFIIH. Importantly, MSL1 depletion leads to decreased phosphorylation of Ser5 of RNA polymerase II. In addition, we demonstrate that MSL1 is a phosphoprotein, and transgenic flies expressing MSL1 phosphomutants show mislocalization of the histone acetyltransferase MOF and histone H4 K16 acetylation, thus ultimately causing male lethality due to a failure of dosage compensation. We propose that, by virtue of its interaction with components of the general transcription machinery, MSL1 exists in different phosphorylation states, thereby modulating transcription in flies.

Details

show
hide
Language(s): eng - English
 Dates: 2016
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/nsmb.3233
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature Structural and Molecular Biology
  Other : Nature Struct Biol
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: New York, NY : Nature Pub. Group
Pages: - Volume / Issue: 23 Sequence Number: - Start / End Page: 580 - 589 Identifier: ISSN: 1545-9993
CoNE: https://pure.mpg.de/cone/journals/resource/954925603763