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  Crystallization and preliminary X-ray analysis of UMP/CMP-kinase from Dictyostelium discoideum with the specific bisubstrate inhibitor P1-(adenosine 5′)-P5-(uridine 5′)-pentaphosphate (UP5A)

Wiesmüller, L., Scheffzek, K., Kliche, W., Goody, R. S., Wittinghofer, A., & Reinstein, J. (1995). Crystallization and preliminary X-ray analysis of UMP/CMP-kinase from Dictyostelium discoideum with the specific bisubstrate inhibitor P1-(adenosine 5′)-P5-(uridine 5′)-pentaphosphate (UP5A). FEBS Letters, 363(1), 22-24. doi:10.1016/0014-5793(95)00271-A.

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Genre: Journal Article
Alternative Title : Crystallization and preliminary X-ray analysis of UMP/CMP-kinase from Dictyostelium discoideum with the specific bisubstrate inhibitor P1-(adenosine 5′)-P5-(uridine 5′)-pentaphosphate (UP5A)

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FEBSLett_363_1995_22.pdf (Any fulltext), 318KB
 
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 Creators:
Wiesmüller, Lisa1, Author              
Scheffzek, Klaus1, Author              
Kliche, Werner2, Author              
Goody, Roger S.1, Author              
Wittinghofer, Alfred1, Author              
Reinstein, Jochen3, Author              
Affiliations:
1Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              
2Emeritus Group Bioorganic Chemistry, Max Planck Institute for Medical Research, Max Planck Society, ou_1497711              
3Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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Free keywords: adenylate kinase; bisubstrate inhibitor; Crystallization; Dictyostelium-discoideum; Ump/cmp-kinase; x-ray analysis
 Abstract: UMP/ICMP-kinase (UK) from the slime mold Dictyostelium discoideum has been purified to high homogeneity and co-crystallized with the bisubstrate inhibitor P1-(adenosine 5′)-P5-(uridine 5′)-pentaphosphate (UP5A). UP5A binds to UK with a dissociation constant (K d ) of 3 ± 0.5 nM at 25°C and pH 7.5. This is some 50-fold tighter than the binding of P1,P5-(diadenosine 5′)-pentaphosphate (AP5A, K d = 160 ± 15 nM). AP5A is a bisubstrate inhibitor that is specific for adenylate kinase. The crystals have the symmetry of the tetragonal space group P41212 or its enantiomorph P43212. The unit cell dimensions are math formula. The crystals diffract to a Bragg spacing of 2.1 Å.

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Language(s): eng - English
 Dates: 1995-02-091995-04-17
 Publication Status: Published in print
 Pages: 3
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 Table of Contents: -
 Rev. Type: Peer
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Title: FEBS Letters
  Other : FEBS Lett.
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 363 (1) Sequence Number: - Start / End Page: 22 - 24 Identifier: ISSN: 0014-5793
CoNE: https://pure.mpg.de/cone/journals/resource/954925399501