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  Determining Substrate Specificities of β1,4-Endo-Galactanases Using Plant Arabinogalactan Oligosaccharides Synthesized by Automated Glycan Assembly

Bartetzko, M. P., Schuhmacher, F., Seeberger, P. H., & Pfrengle, F. (2017). Determining Substrate Specificities of β1,4-Endo-Galactanases Using Plant Arabinogalactan Oligosaccharides Synthesized by Automated Glycan Assembly. The Journal of Organic Chemistry, 82(3), 1842-1850. doi:10.1021/acs.joc.6b02745.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-002C-427E-F Version Permalink: https://hdl.handle.net/11858/00-001M-0000-002D-C1C4-F
Genre: Journal Article

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 Creators:
Bartetzko, Max Peter1, Author           
Schuhmacher, Frank2, Author           
Seeberger, Peter H.3, Author           
Pfrengle, Fabian1, Author           
Affiliations:
1Fabian Pfrengle, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863303              
2Peter H. Seeberger - Automated Systems, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863306              
3Peter H. Seeberger, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_2040285              

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 Abstract: Pectin is a structurally complex plant polysaccharide with many industrial applications in food products. The structural elucidation of pectin is aided by digestion assays with glycosyl hydrolases. We report the automated glycan assembly of oligosaccharides related to the arabinogalactan side chains of pectin as novel biochemical tools to determine the substrate specificities of endo-galactanases. Analysis of the digestion products revealed different requirements for the lengths and arabinose substitution pattern of the oligosaccharides to be recognized and hydrolyzed by the galactanases.

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 Dates: 2017-01-112017
 Publication Status: Issued
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 Identifiers: DOI: 10.1021/acs.joc.6b02745
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Funding program : Emmy Noether Program (PF850/1-1 to F.P. , SFB-765)
Funding organization : DFG

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Title: The Journal of Organic Chemistry
  Other : J. Org. Chem.
Source Genre: Journal
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Publ. Info: Washington, DC : American Chemical Society
Pages: - Volume / Issue: 82 (3) Sequence Number: - Start / End Page: 1842 - 1850 Identifier: ISSN: 0022-3263