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  Structure of the RBM7-ZCCHC8 core of the NEXT complex reveals connections to splicing factors

Falk, S., Finogenova, K., Melko, M., Benda, C., Lykke-Andersen, S., Jensen, T. H., et al. (2016). Structure of the RBM7-ZCCHC8 core of the NEXT complex reveals connections to splicing factors. Nature Communications, 7: 13573. doi:10.1038/ncomms13573.

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 Creators:
Falk, Sebastian1, Author              
Finogenova, Ksenia1, Author              
Melko, Mireille2, Author
Benda, Christian1, Author              
Lykke-Andersen, Soren2, Author
Jensen, Torben Heick2, Author
Conti, Elena1, Author              
Affiliations:
1Conti, Elena / Structural Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565144              
2external, ou_persistent22              

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Free keywords: RNA-QUALITY-CONTROL; EXOSOME TARGETING COMPLEX; SACCHAROMYCES-CEREVISIAE; SKI COMPLEX; POLY(A) POLYMERASE; MESSENGER-RNA; NUCLEAR; YEAST; MOTIF; SURVEILLANCEScience & Technology - Other Topics;
 Abstract: The eukaryotic RNA exosome participates extensively in RNA processing and degradation. In human cells, three accessory factors (RBM7, ZCCHC8 and hMTR4) interact to form the nuclear exosome targeting (NEXT) complex, which directs a subset of non-coding RNAs for exosomal degradation. Here we elucidate how RBM7 is incorporated in the NEXT complex. We identify a proline-rich segment of ZCCHC8 as the interaction site for the RNA-recognition motif (RRM) of RBM7 and present the crystal structure of the corresponding complex at 2.0 resolution. On the basis of the structure, we identify a proline-rich segment within the splicing factor SAP145 with strong similarity to ZCCHC8. We show that this segment of SAP145 not only binds the RRM region of another splicing factor SAP49 but also the RRM of RBM7. These dual interactions of RBM7 with the exosome and the spliceosome suggest a model whereby NEXT might recruit the exosome to degrade intronic RNAs.

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Language(s): eng - English
 Dates: 2016-12-01
 Publication Status: Published online
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000389651300001
DOI: 10.1038/ncomms13573
 Degree: -

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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 7 Sequence Number: 13573 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723